Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-1-14
pubmed:abstractText
The testis-specific histone H1t is synthesized during spermatogenesis exclusively in late pachytene primary spermatocytes. Transcription of the H1t gene is repressed in every tissue except testis. Within the testis, transcription is repressed during development before the spermatocyte stage and in later stages of germinal cell maturation. Mechanisms involved in transcriptional repression of the H1t gene are unknown. To assess the contribution of upstream H1t promoter sequence to transcriptional silencing in nonexpressing cells, H1t-promoted reporter vectors were constructed using pGL3 Basic. Transient expression assays with these reporter vectors driven by H1t promoter deletions allowed us to identify a region from 948 to 780 bp upstream from the H1t transcriptional initiation site that functions as a silencer. Examination of nuclear protein binding to this DNA regulatory region by electrophoretic mobility shift assays using extracts from C127I cells, rat testis, and pachytene spermatocytes revealed a low mobility band produced only by nuclear proteins derived from nonexpressing cells that may contain proteins that repress H1t gene transcription.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0730-2312
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
75
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
555-65
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10572239-Animals, pubmed-meshheading:10572239-Base Sequence, pubmed-meshheading:10572239-Binding, Competitive, pubmed-meshheading:10572239-Binding Sites, pubmed-meshheading:10572239-Cell Line, pubmed-meshheading:10572239-Gene Expression Regulation, pubmed-meshheading:10572239-Gene Silencing, pubmed-meshheading:10572239-Histones, pubmed-meshheading:10572239-Male, pubmed-meshheading:10572239-Mice, pubmed-meshheading:10572239-Molecular Sequence Data, pubmed-meshheading:10572239-Nuclear Proteins, pubmed-meshheading:10572239-Organ Specificity, pubmed-meshheading:10572239-Promoter Regions, Genetic, pubmed-meshheading:10572239-Rats, pubmed-meshheading:10572239-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:10572239-Testis, pubmed-meshheading:10572239-Transcription, Genetic
pubmed:year
1999
pubmed:articleTitle
Binding of nuclear proteins to an upstream element involved in transcriptional regulation of the testis-specific histone H1t gene.
pubmed:affiliation
Research Service (151), Overton Brooks Veterans Administration Medical Center, Shreveport, Louisiana 71101-4295, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, U.S. Gov't, Non-P.H.S.