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rdf:type |
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lifeskim:mentions |
umls-concept:C0017337,
umls-concept:C0022942,
umls-concept:C0025914,
umls-concept:C0026809,
umls-concept:C0079429,
umls-concept:C0086418,
umls-concept:C0086860,
umls-concept:C0179400,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0679058,
umls-concept:C1167622,
umls-concept:C1416661,
umls-concept:C1547699,
umls-concept:C1880022,
umls-concept:C2700640
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pubmed:issue |
24
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pubmed:dateCreated |
2000-1-25
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pubmed:databankReference |
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pubmed:abstractText |
The mouse lactoferrin gene promoter includes a CAAT/GT box, GGGCAATAGGGTGGGGCCAGCCC, which functions as the epidermal growth factor response element (EGFRE) in human endometrial carcinoma RL95-2 cells (RL95). A positive clone, EGFREB, of 2575 bp length, was isolated from an expression library of RL95 cells with a multimer of the EGFRE sequence. In this work, we have identified that EGFREB encodes the C-terminus of Kruppel-like factor 5 (KLF5). This mRNA is most abundant in human colon and small intestine. A full-length cDNA clone was isolated from a human colon library using EGFREB as the hybridization probe. The full-length cDNA consists of 3336 bp with a 302 bp 5'-UTR, a 1663 bp 3'-UTR, and a 1371 bp sequence coding for a 457 amino acid polypeptide. Based on its tissue distribution and sequence homology to the mouse IKLF, we renamed this protein IKLF. DNase I footprinting and electrophoresis mobility shift assay confirmed the binding of IKLF to the EGFRE. The human IKLF gene spans >20 kb in length and is organized into four exons, whose intron/exon junctions follow the GT/AG rule. The three zinc fingers are encoded by three exons. Nuclear localization of IKLF was demonstrated by green fluorescence protein (GFP)-tagged IKLF in transfection experiments and western analysis. Overexpression of IKLF in RL95 cells represses the activity of reporter constructs containing the CAAT/GT box of the mouse lactoferrin gene. These findings imply that IKLF is a nuclear transcription factor that binds to the CAAT/GT box, and functions as a modulator of the mouse lacto-ferrin gene promoter activity.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/KLF5 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Klf5 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Kruppel-Like Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Lactoferrin,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4807-15
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10572182-5' Untranslated Regions,
pubmed-meshheading:10572182-Amino Acid Sequence,
pubmed-meshheading:10572182-Animals,
pubmed-meshheading:10572182-Base Sequence,
pubmed-meshheading:10572182-Binding Sites,
pubmed-meshheading:10572182-Cloning, Molecular,
pubmed-meshheading:10572182-Colon,
pubmed-meshheading:10572182-Endometrial Neoplasms,
pubmed-meshheading:10572182-Exons,
pubmed-meshheading:10572182-Female,
pubmed-meshheading:10572182-Gene Expression Regulation, Neoplastic,
pubmed-meshheading:10572182-Gene Library,
pubmed-meshheading:10572182-Humans,
pubmed-meshheading:10572182-Intestine, Small,
pubmed-meshheading:10572182-Introns,
pubmed-meshheading:10572182-Kruppel-Like Transcription Factors,
pubmed-meshheading:10572182-Lactoferrin,
pubmed-meshheading:10572182-Mice,
pubmed-meshheading:10572182-Molecular Sequence Data,
pubmed-meshheading:10572182-RNA, Messenger,
pubmed-meshheading:10572182-Recombinant Proteins,
pubmed-meshheading:10572182-Sequence Alignment,
pubmed-meshheading:10572182-Sequence Homology, Amino Acid,
pubmed-meshheading:10572182-Sequence Homology, Nucleic Acid,
pubmed-meshheading:10572182-Trans-Activators,
pubmed-meshheading:10572182-Transcription, Genetic,
pubmed-meshheading:10572182-Tumor Cells, Cultured,
pubmed-meshheading:10572182-Zinc Fingers
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pubmed:year |
1999
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pubmed:articleTitle |
Isolation and characterization of a gene encoding human Kruppel-like factor 5 (IKLF): binding to the CAAT/GT box of the mouse lactoferrin gene promoter.
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pubmed:affiliation |
Gene Regulation Group, Laboratory of Reproductive and Development Toxicology, National Institute of Environmental Health Sciences, National Institutes of Health, Research Triangle Park, NC 27709, USA.
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pubmed:publicationType |
Journal Article
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