Linked Life Data

10571538

Source:http://linkedlifedata.com/resource/pubmed/id/10571538

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
2000-1-5
pubmed:abstractText
Action potential duration is prolonged in many forms of heart disease, often as a result of reductions in Ca(2+)-independent transient outward K(+) currents (ie, I(to)). To examine the effects of a primary reduction in I(to) current in the heart, transgenic mice were generated that express a dominant-negative N-terminal fragment of the K(v)4.2 pore-forming potassium channel subunit under the control of the mouse alpha-myosin heavy chain promoter. Two of 6 founders died suddenly, and only 1 mouse successfully transmitted the transgene in mendelian fashion. Electrophysiological analysis at 2 to 4 weeks of age demonstrated that I(to) density was specifically reduced and action potential durations were prolonged in a subset of transgenic myocytes. The heterogeneous reduction in I(to) was accompanied by significant prolongation of monophasic action potentials. In vivo hemodynamic studies at this age revealed significant elevations in the mean arterial pressure, peak systolic ventricular pressures, and +/-dP/dt, indicative of enhanced contractility. Surprisingly, by 10 to 12 weeks of age, transgenic mice developed clinical and hemodynamic evidence of congestive heart failure. Failing transgenic hearts displayed molecular and cellular remodeling, with evidence of hypertrophy, chamber dilatation, and interstitial fibrosis, and individual myocytes showed sharp reductions in I(to) and I(K1) densities, action potential duration prolongation, and increased cell capacitance. Our results confirm that K(v)4.2 subunits contribute to I(to) in the mouse and demonstrate that manipulation of cardiac excitability may secondarily influence contractile performance.
pubmed:commentsCorrections
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0009-7330
pubmed:author
pubmed:issnType
Print
pubmed:day
26
pubmed:volume
85
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1067-76
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10571538-Action Potentials, pubmed-meshheading:10571538-Animals, pubmed-meshheading:10571538-Cardiomegaly, pubmed-meshheading:10571538-Echocardiography, pubmed-meshheading:10571538-Electrocardiography, pubmed-meshheading:10571538-Genes, Dominant, pubmed-meshheading:10571538-Heart Failure, pubmed-meshheading:10571538-Hemodynamics, pubmed-meshheading:10571538-Ion Transport, pubmed-meshheading:10571538-Mice, pubmed-meshheading:10571538-Mice, Inbred CBA, pubmed-meshheading:10571538-Mice, Transgenic, pubmed-meshheading:10571538-Myocardial Contraction, pubmed-meshheading:10571538-Myocardium, pubmed-meshheading:10571538-Myosin Heavy Chains, pubmed-meshheading:10571538-Phenotype, pubmed-meshheading:10571538-Potassium, pubmed-meshheading:10571538-Potassium Channels, pubmed-meshheading:10571538-Potassium Channels, Voltage-Gated, pubmed-meshheading:10571538-Promoter Regions, Genetic, pubmed-meshheading:10571538-Rats, pubmed-meshheading:10571538-Recombinant Fusion Proteins, pubmed-meshheading:10571538-Shal Potassium Channels, pubmed-meshheading:10571538-Ventricular Remodeling
pubmed:year
1999
pubmed:articleTitle
Targeted expression of a dominant-negative K(v)4.2 K(+) channel subunit in the mouse heart.
pubmed:affiliation
Department of Medicine, Division of Cardiology, The Toronto Hospital, University of Toronto, Ontario, Canada.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't