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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
24
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pubmed:dateCreated |
2000-1-6
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pubmed:abstractText |
Terminal deoxynucleotidyl transferase (TdT) catalyzes the addition of nucleotides at the junctions of rearranging Ig and T cell receptor gene segments, thereby generating antigen receptor diversity. Ku is a heterodimeric protein composed of 70- and 86-kDa subunits that binds DNA ends and is required for V(D)J recombination and DNA double-strand break (DSB) repair. We provide evidence for a direct interaction between TdT and Ku proteins. Studies with a baculovirus expression system show that TdT can interact specifically with each of the Ku subunits and with the heterodimer. The interaction between Ku and TdT is also observed in pre-T cells with endogenously expressed proteins. The protein-protein interaction is DNA independent and occurs at physiological salt concentrations. Deletion mutagenesis experiments reveal that the N-terminal region of TdT (131 amino acids) is essential for interaction with the Ku heterodimer. This region, although not important for TdT polymerization activity, contains a BRCA1 C-terminal domain that has been shown to mediate interactions of proteins involved in DNA repair. The induction of DSBs in Cos-7 cells transfected with a human TdT expression construct resulted in the appearance of discrete nuclear foci in which TdT and Ku colocalize. The physical association of TdT with Ku suggests a possible mechanism by which TdT is recruited to the sites of DSBs such as V(D)J recombination intermediates.
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pubmed:grant |
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-10051570,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-10079104,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-1495986,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-2578541,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-3904729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-6300689,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-6572772,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-7699019,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-7892263,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-8082768,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-8163485,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-8422676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-8524303,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-8673121,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-8756676,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-8756720,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9000507,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9097725,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9136882,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9252118,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9305850,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9315668,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9422740,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9430729,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9501103,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9799248,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9813006,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9881976,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10570175-9885240
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Nuclear,
http://linkedlifedata.com/resource/pubmed/chemical/DNA,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Helicases,
http://linkedlifedata.com/resource/pubmed/chemical/DNA Nucleotidylexotransferase,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ku autoantigen,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/XRCC5 protein, human
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0027-8424
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
23
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pubmed:volume |
96
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
13926-31
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10570175-Animals,
pubmed-meshheading:10570175-Antigens, Nuclear,
pubmed-meshheading:10570175-Binding Sites,
pubmed-meshheading:10570175-COS Cells,
pubmed-meshheading:10570175-Cell Line,
pubmed-meshheading:10570175-DNA,
pubmed-meshheading:10570175-DNA Damage,
pubmed-meshheading:10570175-DNA Helicases,
pubmed-meshheading:10570175-DNA Nucleotidylexotransferase,
pubmed-meshheading:10570175-DNA-Binding Proteins,
pubmed-meshheading:10570175-Humans,
pubmed-meshheading:10570175-Nuclear Proteins,
pubmed-meshheading:10570175-Recombinant Fusion Proteins,
pubmed-meshheading:10570175-Spodoptera,
pubmed-meshheading:10570175-Tumor Cells, Cultured
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pubmed:year |
1999
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pubmed:articleTitle |
Association of terminal deoxynucleotidyl transferase with Ku.
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pubmed:affiliation |
University of North Carolina Lineberger Comprehensive Cancer Center, Chapel Hill, NC 27599, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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