Linked Life Data

10564822

Source:http://linkedlifedata.com/resource/pubmed/id/10564822

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-1-11
pubmed:databankReference
pubmed:abstractText
Tumors interact with their environment, reprogramming host cells to induce responses such as angiogenesis, inflammation, immunity and immune suppression. To understand these processes, it is important to identify and isolate new genes whose expression is induced in host tissues in response to tumors. Ascites tumors offer an attractive model for isolating such genes, because responding host peritoneal lining tissues can be cleanly separated from tumor cells growing in suspension within the peritoneal cavity. We here report the cloning by differential display of a novel gene, DLM-1, that is highly up-regulated in the peritoneal lining tissue of mice bearing MOT ascites tumors. Mouse peritoneal macrophages, stimulated by IFN-gamma or LPS, also expressed significant amounts of DLM-1. Up-regulation of DLM-1 became evident by 4h after stimulation with IFN-gamma and was not blocked by cycloheximide, suggesting the presence of IFN responding elements in its transcription regulation region. DLM-1 RNA was detected at significant levels in normal mouse lung, intestinal epithelium, liver and thymus by Northern blot analysis. In situ hybridization of MOT and HT-29 mouse subcutaneous transplanted solid tumors revealed strong DLM-1 expression in the host reactive stromal cells, but not the tumor cells. Sequence analysis of the full-length cDNA clone revealed that it encodes a protein of approx. M(r) 44330 with multiple potential protein kinase C and casein kinase II phosphorylation sites. Our data suggest that DLM-1 plays a role in such important processes as host response in neoplasia.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0378-1119
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
240
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
157-63
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10564822-Amino Acid Sequence, pubmed-meshheading:10564822-Animals, pubmed-meshheading:10564822-Base Sequence, pubmed-meshheading:10564822-Blotting, Northern, pubmed-meshheading:10564822-Cloning, Molecular, pubmed-meshheading:10564822-Cytokines, pubmed-meshheading:10564822-DNA, Complementary, pubmed-meshheading:10564822-DNA-Binding Proteins, pubmed-meshheading:10564822-Female, pubmed-meshheading:10564822-Gene Expression Regulation, pubmed-meshheading:10564822-Glycoproteins, pubmed-meshheading:10564822-Humans, pubmed-meshheading:10564822-In Situ Hybridization, pubmed-meshheading:10564822-Macrophage Activation, pubmed-meshheading:10564822-Macrophages, Peritoneal, pubmed-meshheading:10564822-Male, pubmed-meshheading:10564822-Mice, pubmed-meshheading:10564822-Mice, Inbred Strains, pubmed-meshheading:10564822-Molecular Sequence Data, pubmed-meshheading:10564822-Neoplasm Transplantation, pubmed-meshheading:10564822-Neoplasms, Experimental, pubmed-meshheading:10564822-RNA, Messenger, pubmed-meshheading:10564822-RNA, Neoplasm, pubmed-meshheading:10564822-Sequence Analysis, DNA, pubmed-meshheading:10564822-Tissue Distribution, pubmed-meshheading:10564822-Tumor Cells, Cultured, pubmed-meshheading:10564822-Up-Regulation
pubmed:year
1999
pubmed:articleTitle
Cloning of DLM-1, a novel gene that is up-regulated in activated macrophages, using RNA differential display.
pubmed:affiliation
Department of Pathology, Beth Israel-Deaconess Medical Center and Harvard Medical School, Boston, MA, USA. yfu@caregroup.harvard.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S.