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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5 Pt 1
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pubmed:dateCreated |
1999-12-14
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pubmed:databankReference |
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pubmed:abstractText |
ClC-4 gene was isolated as a putative Cl(-) channel. Due to a lack of functional expression of ClC-4, its physiological role remains unknown. We isolated a human ClC-4 clone (hClC-4sk) from human skeletal muscles and stably transfected it to Chinese hamster ovary cells. Whole cell patch-clamp studies showed that the hClC-4sk channel was activated by external acidic pH and inhibited by DIDS. It passed a strong outward Cl(-) current with a permeability sequence of I(-) > Cl(-) > F(-). The hClC-4sk has consensus sites for phosphorylation by protein kinase A (PKA); however, stimulation of PKA had no effect on the currents. hClC-4sk mRNA was expressed in excitable tissues, such as heart, brain, and skeletal muscle. These functional characteristics of hClC-4sk provide a clue to its physiological role in excitable cells.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/4,4'-Diisothiocyanostilbene-2,2'-Dis...,
http://linkedlifedata.com/resource/pubmed/chemical/Acids,
http://linkedlifedata.com/resource/pubmed/chemical/CLCN4 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Chloride Channels,
http://linkedlifedata.com/resource/pubmed/chemical/Chlorides,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Forskolin
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0002-9513
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
277
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
C948-54
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10564087-4,4'-Diisothiocyanostilbene-2,2'-Disulfonic Acid,
pubmed-meshheading:10564087-Acids,
pubmed-meshheading:10564087-Amino Acid Sequence,
pubmed-meshheading:10564087-Animals,
pubmed-meshheading:10564087-CHO Cells,
pubmed-meshheading:10564087-Chloride Channels,
pubmed-meshheading:10564087-Chlorides,
pubmed-meshheading:10564087-Cloning, Molecular,
pubmed-meshheading:10564087-Cricetinae,
pubmed-meshheading:10564087-DNA, Complementary,
pubmed-meshheading:10564087-Extracellular Space,
pubmed-meshheading:10564087-Forskolin,
pubmed-meshheading:10564087-Gene Expression,
pubmed-meshheading:10564087-Humans,
pubmed-meshheading:10564087-Hydrogen-Ion Concentration,
pubmed-meshheading:10564087-Ion Channel Gating,
pubmed-meshheading:10564087-Mesocricetus,
pubmed-meshheading:10564087-Molecular Sequence Data,
pubmed-meshheading:10564087-Muscle, Skeletal,
pubmed-meshheading:10564087-Patch-Clamp Techniques,
pubmed-meshheading:10564087-Transfection
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pubmed:year |
1999
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pubmed:articleTitle |
Identification of an acid-activated Cl(-) channel from human skeletal muscles.
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pubmed:affiliation |
Second Department of Internal Medicine, Tokyo Medical and Dental University, Tokyo 113-8519, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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