Linked Life Data

10564054

Source:http://linkedlifedata.com/resource/pubmed/id/10564054

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-8-22
pubmed:abstractText
To investigate the role of differentiation of anise tissue cultures on total phenolic and anethole contents, benzylaminopurine- and thidiazuron-induced shoot cultures were generated from roots of the A-8 clonal line and its Agrobacterium rhizogenes-induced genetically transformed derivative JB-10. Embryogenic cultures were induced following 2,4-D treatment. Root cultures were multiplied on hormone-free medium. The effect of proline on differentiation and phenolic synthesis was also investigated. GC/MS studies indicate that anethole was not produced in root or other differentiated cultures. The predominant phenolic metabolite, however, was an anethole precursor, epoxypseudoisoeugenol-2-methylbutyrate (EPB). Total phenolics and EPB contents were highest in root cultures, which also correlated with higher proline content. Embryo and shoot cultures had reduced phenolic level and EPB and proline contents. Antioxidant activity in all differentiating cultures was high on day 60 compared to that on day 30, and there was no significant difference between differentiating tissues. This indicated that antioxidant protection might be linked not only to phenolics but to other nonphenolic metabolites as well.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Apr
pubmed:issn
0021-8561
pubmed:author
pubmed:issnType
Print
pubmed:volume
47
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1776-80
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Phenolic content in differentiated tissue cultures of untransformed and Agrobacterium-transformed roots of anise (Pimpinella anisum L.).
pubmed:affiliation
Department of Food Science, University of Massachusetts, Amherst 01003, USA.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't