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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
2000-1-20
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pubmed:abstractText |
Meiotic development in yeast is characterized by the sequential induction of temporally distinct classes of genes. Genes that are induced at the middle stages of the pathway share a promoter element, termed the middle sporulation element (MSE), which interacts with the Ndt80 transcriptional activator. We have found that a subclass of MSEs are strong repressor sites during mitosis. SUM1 and HST1, genes previously associated with transcriptional silencing, are required for MSE-mediated repression. Sum1 binds specifically in vitro to MSEs that function as strong repressor sites in vivo. Repression by Sum1 is gene specific and does not extend to neighboring genes. These results suggest that mechanisms used to silence large regions of chromatin may also be used to regulate the expression of specific genes during development. NDT80 is regulated during mitosis by both the Sum1 and Ume6 repressors. These results suggest that progression through sporulation may be controlled by the regulated competition between the Sum1 repressor and Ndt80 activator at key MSEs.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fungal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/HST1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Histone Deacetylases,
http://linkedlifedata.com/resource/pubmed/chemical/NDT80 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/SUM1 protein, S cerevisiae,
http://linkedlifedata.com/resource/pubmed/chemical/Saccharomyces cerevisiae Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Sirtuin 2,
http://linkedlifedata.com/resource/pubmed/chemical/Sirtuins,
http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/beta-Galactosidase
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0261-4189
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
18
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
6448-54
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10562556-Binding Sites,
pubmed-meshheading:10562556-DNA-Binding Proteins,
pubmed-meshheading:10562556-Fungal Proteins,
pubmed-meshheading:10562556-Gene Expression Regulation, Fungal,
pubmed-meshheading:10562556-Genotype,
pubmed-meshheading:10562556-Histone Deacetylases,
pubmed-meshheading:10562556-Meiosis,
pubmed-meshheading:10562556-Mitosis,
pubmed-meshheading:10562556-Nuclear Proteins,
pubmed-meshheading:10562556-Promoter Regions, Genetic,
pubmed-meshheading:10562556-Repressor Proteins,
pubmed-meshheading:10562556-Saccharomyces cerevisiae,
pubmed-meshheading:10562556-Saccharomyces cerevisiae Proteins,
pubmed-meshheading:10562556-Sirtuin 2,
pubmed-meshheading:10562556-Sirtuins,
pubmed-meshheading:10562556-Spores, Fungal,
pubmed-meshheading:10562556-Trans-Activators,
pubmed-meshheading:10562556-Transcription Factors,
pubmed-meshheading:10562556-beta-Galactosidase
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pubmed:year |
1999
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pubmed:articleTitle |
Sum1 and Hst1 repress middle sporulation-specific gene expression during mitosis in Saccharomyces cerevisiae.
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pubmed:affiliation |
Waksman Institute and Department of Molecular Biology and Biochemistry, 190 Frelinghuysen Road, Rutgers University, Piscataway, NJ 08854-8020, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
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