Linked Life Data

10561068

Source:http://linkedlifedata.com/resource/pubmed/id/10561068

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-12-7
pubmed:databankReference
pubmed:abstractText
The soybean Ep gene encodes an anionic peroxidase enzyme that accumulates in large amounts in seed coat tissues. We have isolated a second peroxidase gene, Prx2, that is also highly expressed in developing seed coat tissues. Sequence analysis of Prx2 cDNA indicates that this transcript encodes a cationic peroxidase isozyme that is far removed from Ep in peroxidase phylogeny. To determine the expression patterns for these two peroxidases in developing seeds, the abundance and localization of the Ep and Prx2 transcripts were compared by in situ hybridization. Results show the expression of Ep begins in a small number of cells flanking the vascular bundle in the seed coat, spreads to encircle the seed, and then migrates to the hourglass cells as they develop. Expression of Prx2 occurs throughout development in all cell layers of the seed coat, and is also evident in the pericarp and embryo. Nonetheless, the Ep-encoded enzyme accounts for virtually all of the peroxidase activity detected in mature seed coats. The Prx2 enzyme is either insoluble in a catalytically inactive form, or is subject to degradation during seed maturation.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Sep
pubmed:issn
0167-4412
pubmed:author
pubmed:issnType
Print
pubmed:volume
41
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
57-63
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10561068-Blotting, Northern, pubmed-meshheading:10561068-DNA, Complementary, pubmed-meshheading:10561068-Gene Expression Regulation, Developmental, pubmed-meshheading:10561068-Gene Expression Regulation, Enzymologic, pubmed-meshheading:10561068-Gene Expression Regulation, Plant, pubmed-meshheading:10561068-In Situ Hybridization, pubmed-meshheading:10561068-Molecular Sequence Data, pubmed-meshheading:10561068-Peroxidase, pubmed-meshheading:10561068-Peroxidases, pubmed-meshheading:10561068-RNA, Messenger, pubmed-meshheading:10561068-RNA, Plant, pubmed-meshheading:10561068-Seeds, pubmed-meshheading:10561068-Sequence Alignment, pubmed-meshheading:10561068-Sequence Analysis, DNA, pubmed-meshheading:10561068-Soybeans, pubmed-meshheading:10561068-Tissue Distribution, pubmed-meshheading:10561068-Transcription, Genetic
pubmed:year
1999
pubmed:articleTitle
Localization of peroxidase mRNAs in soybean seeds by in situ hybridization.
pubmed:affiliation
Agriculture and Agri-Food Canada, Southern Crop Protection and Food Research Centre, London, Ontario.
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't