|
rdf:type |
|
|
lifeskim:mentions |
|
|
pubmed:issue |
12
|
|
pubmed:dateCreated |
1999-12-20
|
|
pubmed:abstractText |
Previously we demonstrated that murine retroviral Gag proteins associate with a cellular motor protein, KIF-4. Using the yeast two-hybrid assay, we also found an association of KIF-4 with Gag proteins of Mason-Pfizer monkey virus (MPMV), simian immunodeficiency virus (SIV), and human immunodeficiency virus type 1 (HIV-1). Studies performed with mammalian cell systems confirmed that the HIV-1 Gag protein associates with KIF-4. Soluble cytoplasmic proteins from cells infected with recombinant vaccinia virus expressing the entire Gag-Pol precursor protein of HIV-1 or transfected with HIV-1 molecular clone pNL4-3 were fractionated by sucrose gradient centrifugation and further separated by size-exclusion and anion-exchange chromatographies. KIF-4 and HIV-1 Gag cofractionated in both chromatographic separations. Immunoprecipitation assays have also verified the KIF-4-Gag association. KIF-4 binds mainly to the Gag precursor (Pr55 Gag) and a matrix-capsid processing intermediate (Pr42) but not to other processed Gag products. The binding of Gag is mediated by a domain of KIF-4 proximal to the C terminus. These results, and our previous studies, raise the possibility that KIF-4 may play an important role in retrovirus Gag protein transport.
|
|
pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-1883539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-2479031,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-6196908,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-7853546,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-7929562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-8035530,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-8607272,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-8791724,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-8892894,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-9188638,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-9527917,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10559369-9658142
|
|
pubmed:language |
eng
|
|
pubmed:journal |
|
|
pubmed:citationSubset |
IM
|
|
pubmed:chemical |
|
|
pubmed:status |
MEDLINE
|
|
pubmed:month |
Dec
|
|
pubmed:issn |
0022-538X
|
|
pubmed:author |
|
|
pubmed:issnType |
Print
|
|
pubmed:volume |
73
|
|
pubmed:owner |
NLM
|
|
pubmed:authorsComplete |
Y
|
|
pubmed:pagination |
10508-13
|
|
pubmed:dateRevised |
2009-11-18
|
|
pubmed:meshHeading |
pubmed-meshheading:10559369-Animals,
pubmed-meshheading:10559369-Avian Sarcoma Viruses,
pubmed-meshheading:10559369-Gene Products, gag,
pubmed-meshheading:10559369-HIV-1,
pubmed-meshheading:10559369-HeLa Cells,
pubmed-meshheading:10559369-Humans,
pubmed-meshheading:10559369-Kinesin,
pubmed-meshheading:10559369-Mason-Pfizer monkey virus,
pubmed-meshheading:10559369-Precipitin Tests,
pubmed-meshheading:10559369-Recombinant Fusion Proteins,
pubmed-meshheading:10559369-Retroviridae,
pubmed-meshheading:10559369-Simian immunodeficiency virus
|
|
pubmed:year |
1999
|
|
pubmed:articleTitle |
Cellular motor protein KIF-4 associates with retroviral Gag.
|
|
pubmed:affiliation |
Laboratory of Immunopathology, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.
|
|
pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|
