Linked Life Data

10558862

Source:http://linkedlifedata.com/resource/pubmed/id/10558862

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
2
pubmed:dateCreated
1999-12-20
pubmed:abstractText
A shift down in temperature causes in Streptomyces aureofaciens a transient repression of polypeptide synthesis. During the acclimation phase 32 proteins were synthesized. The addition of tetracycline (200 microg/ml) to cells from exponential phase of growth leads to induction of 27 novel proteins and 17 upregulated proteins migrated in 2-D gel as proteins expressed upon cold shock. Immunoblot analysis using antibodies raised against CspB, CspC, and CspD of Bacillus subtilis revealed five cross-reactive proteins of the Csp family. Proteins CspB and CspD are predominantly induced at low temperature or by the presence of tetracycline. Expression of Csp proteins during the acclimation phase is regulated on the transcription level. Proteins of the Csp family have been shown to be associated with ribosomes and can be removed by 1 M NH(4)Cl. As expression of Csp proteins differs during development or temperature shift down, these proteins can be considered as trans-acting factors to form contacts with the coding region of specific mRNAs.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0006-291X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
19
pubmed:volume
265
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
305-10
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Expression of the Csp protein family upon cold shock and production of tetracycline in Streptomyces aureofaciens.
pubmed:affiliation
Institute of Microbiology, Academy of Sciences of the Czech Republic, Vídenská 1083, Prague, 4, 142 20, Czech Republic. mikulik@biomed.cas.cz
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't