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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1999-12-14
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pubmed:abstractText |
1 Guanethidine is commonly used as a drug to investigate adrenergic neurotransmission and, in combination with atropine, to realize non-adrenergic non-cholinergic (NANC) conditions. Previous studies suggested a nicotinic acetylcholine receptor blocking effect of guanethidine. Therefore, we investigated the effect of increasing concentrations of guanethidine (0.1-100 microM) on nicotine-induced relaxations of longitudinal muscle strips of rat gastric fundus. 2 In the presence of 1 microM atropine and 3 microM guanethidine, nicotine (30 microM) induces a fast and sustained relaxation which is partly inhibited by the nitric oxide synthase inhibitors Nomega-nitro-L-arginine (L-NOARG) and Nomega-nitro-L-arginine methyl ester (L-NAME) (both 30 and 100 microM). One microM tetrodotoxin (TTX) completely blocks this nicotine-induced relaxation. 3 High concentrations of guanethidine (> or =10 microM), but not adrenoceptor blockade by the alpha-adrenoceptor antagonist phentolamine in combination with the beta-adrenoceptor antagonist nadolol (both 3 microM), inhibit the nicotine-induced relaxation. 4 Guanethidine (0.1-100 microM) has no effect on relaxations induced by electrical field stimulation (EFS; 1-8 Hz), nitric oxide (NO; 0.01-1 microM), vasoactive intestinal polypeptide (VIP; 0.1-10 nM) or isoprenaline (1-10 nM). 5 We conclude that high concentrations of guanethidine (> or =10 microM) block nicotine-induced NANC relaxations of longitudinal muscle strips of the rat gastric fundus most likely at the level of the nicotinic acetylcholine receptor.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-14269604,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-1463368,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-1652335,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-1671594,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-1964906,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-3260776,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-3524491,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-7602539,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-7678206,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-8339469,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-8387048,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-8879538,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556924-9249245
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0007-1188
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
128
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
903-8
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10556924-Animals,
pubmed-meshheading:10556924-Electric Stimulation,
pubmed-meshheading:10556924-Gastric Fundus,
pubmed-meshheading:10556924-Guanethidine,
pubmed-meshheading:10556924-Hexamethonium,
pubmed-meshheading:10556924-Isoproterenol,
pubmed-meshheading:10556924-Male,
pubmed-meshheading:10556924-Muscle Relaxation,
pubmed-meshheading:10556924-Nicotinic Antagonists,
pubmed-meshheading:10556924-Nitric Oxide,
pubmed-meshheading:10556924-Nitric Oxide Synthase,
pubmed-meshheading:10556924-Rats,
pubmed-meshheading:10556924-Rats, Wistar,
pubmed-meshheading:10556924-Receptors, Nicotinic,
pubmed-meshheading:10556924-Tetrodotoxin,
pubmed-meshheading:10556924-Vasoactive Intestinal Peptide
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pubmed:year |
1999
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pubmed:articleTitle |
Nicotinic acetylcholine receptor blocking effect of guanethidine in the rat gastric fundus.
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pubmed:affiliation |
Division of Gastroenterology and Hepatology, Academic Medical Center, Amsterdam, The Netherlands.
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pubmed:publicationType |
Journal Article,
In Vitro
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