Linked Life Data

10556919

Source:http://linkedlifedata.com/resource/pubmed/id/10556919

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-12-14
pubmed:abstractText
1 The localization of protease-activated receptor-2 (PAR2) and the effects of PAR2 activators were investigated in the mouse isolated ureter in order to test the hypothesis that PAR2 activation may initiate neuropeptide release from sensory nerve fibres and hence contribute to inflammation. 2 PAR2 was localized by fluorescence immunohistochemistry to both the smooth muscle and epithelium of the ureter. Macrophage-like cells in the adventitia of the ureter were also PAR2-immunoreactive. PAR2-immunoreactivity was not observed in mast cells or nerve fibres. 3 In circular muscle preparations of the ureter in which continuous rhythmic beating was induced by KCl (20 mM) and the thromboxane A2 mimetic U46619 (0.3 microM), trypsin (0.3 U ml-1) reduced beat frequency to 84.6+/-2.0% of control rates. The PAR2-selective peptide agonist SLIGRL-NH2 concentration-dependently (0.1-3.0 microM) slowed beat frequency to a maximum of 72.7+/-2.0%. 4 Histamine (1-300 microM) was more efficacious than SLIGRL-NH2 in inhibiting ureter beat frequency in a concentration-dependent manner to a maximum (at 300 microM) of 7.9+/-2.5% of the control rate. 5 Pretreatment of preparations with capsaicin (10 microM for 30 min) markedly attenuated the inhibitory effect of histamine, but not that of SLIGRL-NH2, indicating a role for sensory nerves in the inhibitory effect of histamine only. 6 The inhibitory effect of SLIGRL-NH2 on ureter beat frequency was unaffected by the nitric oxide (NO) synthase inhibitor, L-NOARG (100 microM) or the cyclo-oxygenase inhibitor, indomethacin (3 microM). 7 In conclusion, PAR2 activation causes inhibition of beating in the mouse ureter that is not mediated by axon reflex release of inhibitory neuropeptides. This inhibitory effect of PAR2 appears to be mediated directly on smooth muscle cells, although the contribution of non-NO, non-prostanoid epithelium-derived factors cannot be ruled out.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-10029617, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-10086357, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-1672265, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-1952556, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-8635215, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-8685245, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-8762073, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-8832077, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-8846429, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9130474, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9272619, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9409730, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9446822, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9598843, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9618465, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9636225, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9655871, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9660401, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9696685, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9806321, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9831889, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9860807, http://linkedlifedata.com/resource/pubmed/commentcorrection/10556919-9884072
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0007-1188
pubmed:author
pubmed:issnType
Print
pubmed:volume
128
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
860-4
pubmed:dateRevised
2010-9-13
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
The role of protease-activated receptor-2 (PAR2) in the modulation of beating of the mouse isolated ureter: lack of involvement of mast cells or sensory nerves.
pubmed:affiliation
Department of Pharmacology, Triradiate Building, The University of Melbourne, Parkville, Victoria 3052, Australia. james_moffatt@hotmail.com
pubmed:publicationType
Journal Article, In Vitro, Research Support, Non-U.S. Gov't