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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
11
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pubmed:dateCreated |
1999-12-23
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pubmed:abstractText |
Using the monoclonal antibody C1.7, which recognizes a signaling, membrane-bound molecule on human NK and a proportion of CD8(+) T cells, we cloned a novel molecule we refer to as NK cell activation-inducing ligand (NAIL). It is a 365-amino acid protein that belongs to the immunoglobulin-like superfamily with closest homology to murine 2B4, and human CD84 and CD48. Using a soluble NAIL-Fc fusion protein, we determined the counterstructure for NAIL, CD48, which it binds with high affinity. Stimulation of human B cells with recombinant NAIL in the presence of a suboptimal concentration of human CD40 ligand or IL-4 resulted in increased proliferation. Treatment of human dendritic cells with soluble NAIL-leucine zipper protein resulted in an increased release of IL-12 and TNF-alpha. Using recombinant CD48 protein, we demonstrated the ability of this molecule to increase NK cell cytotoxicity and induce IFN-gamma production. We also showed that 2B4 binds to mouse CD48, suggesting that interaction of these receptors may play a similar role in both species. Taken together these results indicate that the NAIL-CD48 interaction may be an important mechanism regulating a variety of immune responses.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/CD244 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/CD48 antigen,
http://linkedlifedata.com/resource/pubmed/chemical/Cd244 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Cytokines,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin Fc Fragments,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Immunologic,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Tyrosine
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0014-2980
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pubmed:author |
pubmed-author:ArmitageR JRJ,
pubmed-author:BorgesLL,
pubmed-author:CassianoLL,
pubmed-author:ChinWW,
pubmed-author:Davis-SmithTT,
pubmed-author:DinWW,
pubmed-author:GoodwinR GRG,
pubmed-author:KuninI JIJ,
pubmed-author:MacduffB MBM,
pubmed-author:ParshleyD LDL,
pubmed-author:PetersenMM,
pubmed-author:SmithC ACA,
pubmed-author:TrinchieriGG,
pubmed-author:WaughJ YJY
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pubmed:issnType |
Print
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pubmed:volume |
29
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
3466-77
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10556801-Amino Acid Sequence,
pubmed-meshheading:10556801-Animals,
pubmed-meshheading:10556801-Antigens, CD,
pubmed-meshheading:10556801-B-Lymphocytes,
pubmed-meshheading:10556801-Cell Division,
pubmed-meshheading:10556801-Cloning, Molecular,
pubmed-meshheading:10556801-Cytokines,
pubmed-meshheading:10556801-Cytotoxicity, Immunologic,
pubmed-meshheading:10556801-Humans,
pubmed-meshheading:10556801-Immunoglobulin Fc Fragments,
pubmed-meshheading:10556801-Interferon-gamma,
pubmed-meshheading:10556801-Jurkat Cells,
pubmed-meshheading:10556801-K562 Cells,
pubmed-meshheading:10556801-Killer Cells, Natural,
pubmed-meshheading:10556801-Ligands,
pubmed-meshheading:10556801-Membrane Glycoproteins,
pubmed-meshheading:10556801-Membrane Proteins,
pubmed-meshheading:10556801-Mice,
pubmed-meshheading:10556801-Molecular Sequence Data,
pubmed-meshheading:10556801-Phosphorylation,
pubmed-meshheading:10556801-RNA, Messenger,
pubmed-meshheading:10556801-Receptors, Immunologic,
pubmed-meshheading:10556801-Recombinant Fusion Proteins,
pubmed-meshheading:10556801-Tyrosine,
pubmed-meshheading:10556801-U937 Cells,
pubmed-meshheading:10556801-Up-Regulation
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pubmed:year |
1999
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pubmed:articleTitle |
Molecular cloning and biological characterization of NK cell activation-inducing ligand, a counterstructure for CD48.
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pubmed:affiliation |
Department of Immunobiology, Immunex Corporation, Seattle, USA. mkubin@immunex.com
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
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