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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
2000-1-11
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pubmed:abstractText |
Within the expressed sequence tag (EST) dataset of Toxoplasma gondii we have identified several ESTs encoding a protein similar to the small zinc finger protein MPS1. In human it is suggested that MPS1 plays a role as a transcriptional mediator in response to various growth factors and it is used as a tumour marker in sera from cancer patients. However, in rat a cDNA sequence homologous to MPS1 encodes ribosomal protein S27. To further characterise MPS1 in T. gondii we transformed tachyzoites with a c-Myc-tagged version of the Toxoplasma MPS1 cDNA, flanked by SAG1 sequences. Western blot analysis showed that the Myc-MPS1 was only poorly expressed in the stable transformants. In contrast, Northern blot analysis demonstrated that the Myc-MPS1 mRNA was abundantly transcribed and that the endogenous level of MPS1 mRNA was not affected.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Metalloproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protozoan Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/RPS27 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Ribosomal Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Rps27 protein, rat
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0378-1097
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
180
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
235-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10556717-Amino Acid Sequence,
pubmed-meshheading:10556717-Animals,
pubmed-meshheading:10556717-Blotting, Northern,
pubmed-meshheading:10556717-Blotting, Western,
pubmed-meshheading:10556717-Cloning, Molecular,
pubmed-meshheading:10556717-DNA, Complementary,
pubmed-meshheading:10556717-Humans,
pubmed-meshheading:10556717-Metalloproteins,
pubmed-meshheading:10556717-Molecular Sequence Data,
pubmed-meshheading:10556717-Nuclear Proteins,
pubmed-meshheading:10556717-Protozoan Proteins,
pubmed-meshheading:10556717-RNA-Binding Proteins,
pubmed-meshheading:10556717-Rats,
pubmed-meshheading:10556717-Recombinant Proteins,
pubmed-meshheading:10556717-Ribosomal Proteins,
pubmed-meshheading:10556717-Sequence Alignment,
pubmed-meshheading:10556717-Toxoplasma,
pubmed-meshheading:10556717-Transfection,
pubmed-meshheading:10556717-Zinc Fingers
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pubmed:year |
1999
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pubmed:articleTitle |
MPS1: a small, evolutionarily conserved zinc finger protein from the protozoan Toxoplasma gondii.
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pubmed:affiliation |
ZMBH, University of Heidelberg, Im Neuenheimer Feld 282, D-69120, Heidelberg, Germany. jens.mattsson@sva.se
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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