Linked Life Data

10556314

Source:http://linkedlifedata.com/resource/pubmed/id/10556314

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
23
pubmed:dateCreated
2000-1-14
pubmed:abstractText
An easy and routine procedure to amplify messenger RNA (mRNA) libraries from a few tissue cells can provide molecular gene expression profiles at high resolution. A novel PCR-like method, the RNA-PCR, was developed to generate high quality and quantity mRNAs from as few as 20 cells (2 pg mRNAs). The principle relies upon the cycling steps of promoter-linked double-stranded cDNA synthesis and promoter-driven transcription to amplify mRNAs up to 250-fold/cycle with good representation of high and low copy mRNAs. The amplified mRNA libraries were shown to possess high fidelity, purity, specificity and reproducibility for in vivo analyses of cancerous gene expression in human prostate cancers.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0305-1048
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
27
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4585-9
pubmed:dateRevised
2011-3-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
In vivo analysis of cancerous gene expression by RNA-polymerase chain reaction.
pubmed:affiliation
Department of Pathology, University of Southern California School of Medicine, HMR-209, 2011 Zonal Avenue, Los Angeles, CA 90033, USA. shilungl@hsc.usc.edu
pubmed:publicationType
Journal Article