Linked Life Data

10554023

Source:http://linkedlifedata.com/resource/pubmed/id/10554023

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
21
pubmed:dateCreated
1999-12-2
pubmed:abstractText
The introduction of a discontinuous approximately 70-cM portion of human chromosome 17 significantly suppresses the metastatic ability of AT6.1 rat prostate cancer cells without affecting tumorigenicity (M. A. Chekmareva et al., Prostate, 33: 271-280, 1997). We have recently demonstrated that AT6.1 cells containing the approximately 70-cM region (AT6.1-17-4 cells) escape from the primary tumor and arrest in the lung but are growth-inhibited unless the metastasis suppressor region is lost (M. A. Chekmareva et al., Cancer Res., 58: 4963-4969, 1998). A series of in vivo studies indicated that the observed growth inhibition was due to the effect of a gene(s) at the metastatic site (M. A. Chekmareva et al., Cancer Res., 58: 4963-4969, 1998). We have now identified the mitogen-activated protein kinase kinase 4/stress-activated protein/Erk kinase 1 (MKK4/SEK1) gene as a candidate metastasis suppressor gene encoded by the approximately 70-cM region. AT6.1 cells were transfected with a MKK4/SEK1 expression construct, and the cells were tested in standard spontaneous metastasis assays. Whereas the metastatic ability of the AT6.1-MKK4/SEK1 cells was significantly reduced as compared with that of transfection controls, the growth rate of the primary tumors was not affected; the average tumor volume at day 29 after injection was approximately 2 cm. Furthermore, histological examination of the lungs of AT6.1-MKK4/SEK1 tumor-bearing animals revealed that the suppression by MKK4/SEK1 is due to an effect at the metastatic site, consistent with the phenotype conferred by the original approximately 70-cM chromosomal region. These studies implicate MKK4/SEK1 as a metastasis suppressor gene encoded by human chromosome 17.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0008-5472
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
5483-7
pubmed:dateRevised
2009-11-19
pubmed:meshHeading
pubmed-meshheading:10554023-Animals, pubmed-meshheading:10554023-Chromosomes, Human, Pair 17, pubmed-meshheading:10554023-DNA, Complementary, pubmed-meshheading:10554023-Genes, Tumor Suppressor, pubmed-meshheading:10554023-Humans, pubmed-meshheading:10554023-Lung, pubmed-meshheading:10554023-MAP Kinase Kinase 4, pubmed-meshheading:10554023-Male, pubmed-meshheading:10554023-Mice, pubmed-meshheading:10554023-Mice, SCID, pubmed-meshheading:10554023-Mitogen-Activated Protein Kinase Kinases, pubmed-meshheading:10554023-Neoplasm Metastasis, pubmed-meshheading:10554023-Phenotype, pubmed-meshheading:10554023-Prostatic Neoplasms, pubmed-meshheading:10554023-Protein-Tyrosine Kinases, pubmed-meshheading:10554023-Rats, pubmed-meshheading:10554023-Transfection, pubmed-meshheading:10554023-Tumor Cells, Cultured
pubmed:year
1999
pubmed:articleTitle
Mitogen-activated protein kinase kinase 4/stress-activated protein/Erk kinase 1 (MKK4/SEK1), a prostate cancer metastasis suppressor gene encoded by human chromosome 17.
pubmed:affiliation
Department of Surgery, University of Chicago, Illinois 60637, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't