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pubmed-article:10553162pubmed:abstractTextEwing's tumors (ET) are primary malignancies of bone and soft tissues characterized in at least 96% of cases by specific fusion transcripts originating from recurrent chromosomal translocations. Clinical protocols for high-risk metastatic ETs include high-dose radiation/chemotherapy followed by autologous peripheral blood stem cell (PBSC) reinfusion. We used nested reverse transcriptase polymerase chain reaction (RT-PCR) to search for the presence of ET-specific transcripts in PBSC collections from patients with high-risk ET in order to collect harvests free from neoplastic cells but still sufficient to obtain early stable engraftment.lld:pubmed
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pubmed-article:10553162pubmed:dateRevised2011-11-17lld:pubmed
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pubmed-article:10553162pubmed:articleTitleDetection of EWS chimeric transcripts by nested RT-PCR to allow reinfusion of uncontaminated peripheral blood stem cells in high-risk Ewing's tumor in childhood.lld:pubmed
pubmed-article:10553162pubmed:affiliationServizio di Citopatologia; Clinica Pediatrica, Università degli Studi di Bologna, Policlinico S. Orsola, Bologna, Italy.lld:pubmed
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