Source:http://linkedlifedata.com/resource/pubmed/id/10553086
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions |
umls-concept:C0034700,
umls-concept:C0039194,
umls-concept:C0085358,
umls-concept:C0205087,
umls-concept:C0458827,
umls-concept:C0700624,
umls-concept:C0871261,
umls-concept:C1332717,
umls-concept:C1413244,
umls-concept:C1704632,
umls-concept:C1706438,
umls-concept:C1706817,
umls-concept:C2698600,
umls-concept:C2911692
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| pubmed:issue |
10
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| pubmed:dateCreated |
1999-12-2
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| pubmed:abstractText |
To test the hypothesis that CD8+ T cells may suppress the allergen-induced late airway response (LAR) and airway eosinophilia, we examined the effect of administration of Ag-primed CD8+ T cells on allergic airway responses, bronchoalveolar lavage (BAL) leukocytes, and mRNA expression for cytokines (IL-4, IL-5, and IFN-gamma) in OVA-sensitized Brown Norway rats. On day 12 postsensitization to OVA, test rats were administered 2 million CD8+ T cells i.p. isolated from either the cervical lymph nodes (LN group; n = 8) or the spleen (Spl group; n = 6) of sensitized donors. On day 14, test rats were challenged with aerosolized OVA. Control rats were administered PBS i.p. on day 12, and challenged with OVA (n = 10) or BSA (n = 6) on day 14. The lung resistance was measured for 8 h after challenge. BAL was performed at 8 h. Cytospin slides of BAL were analyzed for major basic protein by immunostaining and for cytokine mRNA by in situ hybridization. The LAR was significantly less in the LN group (1.8 +/- 0.5 U; p < 0.01) and BSA controls (1.4 +/- 0.7; p < 0.01), but not in the Spl group (6.7 +/- 2.2), compared with that in OVA controls (8.1 +/- 1.8). In BAL, the number of major basic protein-positive cells was lower in the LN and Spl groups compared with OVA controls (p < 0.05 and p < 0.01). IL-4- and IL-5-positive cells were decreased in the LN group compared with the OVA controls (p < 0.01). INF-gamma-positive cells were increased in the LN and Spl groups compared with the OVA controls (p < 0.01). Serum OVA-specific IgE levels were unaffected by CD8+ T cell transfers. These results indicate that Ag-primed CD8+ T cells have a potent suppressive effect on LAR.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
15
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| pubmed:volume |
163
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
5574-81
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10553086-Adoptive Transfer,
pubmed-meshheading:10553086-Animals,
pubmed-meshheading:10553086-Bronchial Provocation Tests,
pubmed-meshheading:10553086-Bronchoalveolar Lavage Fluid,
pubmed-meshheading:10553086-CD8-Positive T-Lymphocytes,
pubmed-meshheading:10553086-Cytokines,
pubmed-meshheading:10553086-Flow Cytometry,
pubmed-meshheading:10553086-Hypersensitivity, Delayed,
pubmed-meshheading:10553086-Immunoglobulin E,
pubmed-meshheading:10553086-Leukocytes, Mononuclear,
pubmed-meshheading:10553086-Lymph Nodes,
pubmed-meshheading:10553086-Lymphocyte Count,
pubmed-meshheading:10553086-Lymphocyte Subsets,
pubmed-meshheading:10553086-Male,
pubmed-meshheading:10553086-Ovalbumin,
pubmed-meshheading:10553086-Rats,
pubmed-meshheading:10553086-Rats, Inbred BN,
pubmed-meshheading:10553086-Spleen
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| pubmed:year |
1999
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| pubmed:articleTitle |
CD8+ T cells modulate late allergic airway responses in Brown Norway rats.
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| pubmed:affiliation |
Meakins-Christie Laboratories, Royal Victoria Hospital, McGill University, Montreal, Quebec, Canada.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
|