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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
10
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pubmed:dateCreated |
1999-12-2
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pubmed:abstractText |
Immunoregulation of lymphocytes and macrophages in the peripheral immune system is achieved in part by activation-induced cell death. Members of the TNF receptor family including Fas (CD95) are involved in the regulation of activation-induced cell death. To determine whether activation-induced cell death plays a role in regulation of dendritic cells (DCs), we examined interactions between Ag-presenting murine DCs and Ag-specific Th1 CD4+ T cells. Whereas mature bone marrow- or spleen-derived DCs expressed high levels of Fas, these DCs were relatively insensitive to Fas-mediated killing by the agonist mAb, Jo-2, as well as authentic Fas ligand expressed on the CD4+ T cell line, A.E7. The insensitivity to Fas-mediated apoptosis was not affected by priming with IFN-gamma and/or TNF-alpha or by blocking the DC survival signals TNF-related activation-induced cytokine and CD40L. However, apoptosis could be induced with C2-ceramide, suggesting that signals proximal to the generation of ceramide might mediate resistance to Fas. Analysis of protein expression of several anti-apoptotic mediators revealed that expression of the intracellular inhibitor of apoptosis Fas-associated death domain-like IL-1-converting enzyme-inhibitory protein was significantly higher in Fas-resistant DCs than in Fas-sensitive macrophages, suggesting a possible role for Fas-associated death domain-like IL-1-converting enzyme-inhibitory protein in DC resistance to Fas-mediated apoptosis. Our results demonstrate that murine DCs differ significantly from other APC populations in susceptibility to Fas-mediated apoptosis during cognate presentation of Ag. Because DCs are most notable for initiation of an immune response, resistance to apoptosis may contribute to this function.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD40,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD95,
http://linkedlifedata.com/resource/pubmed/chemical/CD40 Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Fas Ligand Protein,
http://linkedlifedata.com/resource/pubmed/chemical/Fasl protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Granulocyte-Macrophage...,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/RANK Ligand,
http://linkedlifedata.com/resource/pubmed/chemical/Receptor Activator of Nuclear...,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfrsf11a protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Tnfsf11 protein, mouse
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
15
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5303-11
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10553053-Animals,
pubmed-meshheading:10553053-Antigens, CD40,
pubmed-meshheading:10553053-Antigens, CD95,
pubmed-meshheading:10553053-Apoptosis,
pubmed-meshheading:10553053-Bone Marrow Cells,
pubmed-meshheading:10553053-CD40 Ligand,
pubmed-meshheading:10553053-Carrier Proteins,
pubmed-meshheading:10553053-Cell Differentiation,
pubmed-meshheading:10553053-Cell Line,
pubmed-meshheading:10553053-Dendritic Cells,
pubmed-meshheading:10553053-Fas Ligand Protein,
pubmed-meshheading:10553053-Granulocyte-Macrophage Colony-Stimulating Factor,
pubmed-meshheading:10553053-Immunity, Innate,
pubmed-meshheading:10553053-Ligands,
pubmed-meshheading:10553053-Macrophages,
pubmed-meshheading:10553053-Membrane Glycoproteins,
pubmed-meshheading:10553053-Mice,
pubmed-meshheading:10553053-Mice, Inbred BALB C,
pubmed-meshheading:10553053-Mice, Inbred C3H,
pubmed-meshheading:10553053-Mice, Inbred C57BL,
pubmed-meshheading:10553053-Mice, Inbred MRL lpr,
pubmed-meshheading:10553053-Mice, Mutant Strains,
pubmed-meshheading:10553053-RANK Ligand,
pubmed-meshheading:10553053-Receptor Activator of Nuclear Factor-kappa B,
pubmed-meshheading:10553053-Signal Transduction,
pubmed-meshheading:10553053-Spleen,
pubmed-meshheading:10553053-Th1 Cells,
pubmed-meshheading:10553053-Up-Regulation
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pubmed:year |
1999
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pubmed:articleTitle |
Dendritic cells are resistant to apoptosis through the Fas (CD95/APO-1) pathway.
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pubmed:affiliation |
Hospital for Special Surgery, Cornell University Medical Center, New York 10021, USA. ashanyd@hss.edu
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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