Source:http://linkedlifedata.com/resource/pubmed/id/10552931
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
3
|
| pubmed:dateCreated |
2000-2-14
|
| pubmed:databankReference | |
| pubmed:abstractText |
Gas7, a growth arrest-specific gene first isolated from serum-starved NIH3T3 cells, is expressed abundantly in the brain and is essential for the outgrowth of neurites from cultured cerebellar neurons. Here, we report the existence of a Gas7-related cDNA, designated Gas7-cb, isolated from the mouse cerebellum, and we report the finding that Gas7-cb transcripts and protein are expressed at different locations than those of Gas7. Gas7-cb cDNA differs from the Gas7 cDNA only in the 5' region. Its encoded protein shares the same 320 amino acids in its C-terminus with those of Gas7. Analyses of the RNA and protein expression of Gas7-cb and Gas7 by RNase protection assay and Western blot indicated that while Gas7 expression is predominant in the cerebrum and in growth-arrested NIH3T3 fibroblasts, Gas7-cb expression is predominant in the cerebellum. Characterization of Gas7 and Gas7-cb RNAs and of the genomic structure of murine Gas7 cloned in a bacterial artificial chromosome indicated that the Gas7 gene spans more than 60 kb and consists of at least 15 exons. The 5'-terminus of Gas7-cb is located at exon 6a, which is absent in Gas7 transcripts but is retained in its entirety in Gas7-cb transcripts, resulting in the presence of a unique 20-amino-acid sequence at the N-terminus of the Gas7-cb protein. Our results show that the Gas7 gene encodes two Gas7 isoforms, Gas7 and Gas7-cb, whose expression is differentially regulated within mouse brain.
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| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/5' Untranslated Regions,
http://linkedlifedata.com/resource/pubmed/chemical/DNA, Complementary,
http://linkedlifedata.com/resource/pubmed/chemical/Gas7 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms,
http://linkedlifedata.com/resource/pubmed/chemical/Ribonucleases
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0888-7543
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| pubmed:author | |
| pubmed:copyrightInfo |
Copyright 1999 Academic Press.
|
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
61
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
298-306
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| pubmed:dateRevised |
2006-11-15
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| pubmed:meshHeading |
pubmed-meshheading:10552931-3T3 Cells,
pubmed-meshheading:10552931-5' Untranslated Regions,
pubmed-meshheading:10552931-Animals,
pubmed-meshheading:10552931-Base Sequence,
pubmed-meshheading:10552931-Blotting, Southern,
pubmed-meshheading:10552931-Blotting, Western,
pubmed-meshheading:10552931-Brain,
pubmed-meshheading:10552931-Chromosome Mapping,
pubmed-meshheading:10552931-DNA, Complementary,
pubmed-meshheading:10552931-Exons,
pubmed-meshheading:10552931-Gene Expression Profiling,
pubmed-meshheading:10552931-Gene Expression Regulation,
pubmed-meshheading:10552931-Mice,
pubmed-meshheading:10552931-Molecular Sequence Data,
pubmed-meshheading:10552931-Nerve Tissue Proteins,
pubmed-meshheading:10552931-Protein Isoforms,
pubmed-meshheading:10552931-Ribonucleases,
pubmed-meshheading:10552931-Sequence Analysis, DNA
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| pubmed:year |
1999
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| pubmed:articleTitle |
The Gas7 gene encodes two protein isoforms differentially expressed within the brain.
|
| pubmed:affiliation |
Institute of Molecular Biology, Academia Sinica, Nankang, 115, Taiwan.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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