Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
1999-11-30
pubmed:abstractText
Two isoforms of the human cadherin-11/OB-cadherin gene, the intact and the variant forms, had been isolated from an osteosarcoma cDNA library. The intact form has a typical cadherin structure, whereas the variant form, generated by alternative splicing, encodes a cytoplasmic domain that is completely different from that of the intact form and lacks a homophilic cell-cell adhesion ability. At the protein level, the secreted form generated from the intact cadherin-11 is present. We examined the expression of the intact and the variant forms of cadherin-11 in 23 primary and metastatic osteosarcomas from 22 patients by reverse transcriptase-polymerase chain reaction (RT-PCR) analyses, revealing that all 23 tumors in the patients expressed the variant form and three of them expressed it prominently. On the other hand, Western blot analyses of six tumors showed that the secreted form was strongly expressed, and furthermore, expression of N-cadherin was extremely low. Overexpression of the intact cadherin-11 cDNA in osteosarcoma cell lines demonstrated that the secreted form is derived from the intact form of cadherin-11 in osteosarcoma. Immunohistochemically, cadherin-11, N-cadherin, and beta-catenin were expressed at the cell surface of fetal osteoblasts, whereas in osteosarcoma cells, they were expressed only focally or weakly in the cytoplasm. Considering the function of cadherin in carcinomas, it is suggested that the anomalous expression of human cadherin-11 in osteosarcoma and the reduced expression of N-cadherin play a role in metastasis and the irregular morphology in the highly malignant mesenchymal tumor.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-10320525, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-1419062, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-2006419, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-2078434, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-3611200, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-7750649, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-7750650, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-7862112, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-7982033, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-8123491, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-8127895, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-8163513, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-8616818, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-8658214, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-8725287, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-9412478, http://linkedlifedata.com/resource/pubmed/commentcorrection/10550312-9556063
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0002-9440
pubmed:author
pubmed:issnType
Print
pubmed:volume
155
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
1549-55
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Anomalous cadherin expression in osteosarcoma. Possible relationships to metastasis and morphogenesis.
pubmed:affiliation
Department of Pathology, Faculty of Medicine, Institute for Medical Science, University of Tokyo, Tokyo Japan.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't