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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1999-11-23
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pubmed:abstractText |
Naive Itk-deficient CD4+ T cells were unable to establish stable IL-4 production, even when primed in Th2-inducing conditions. In contrast, IFNgamma production was little affected. Failure to express IL-4 occurred even among cells that had gone through multiple cell divisions and was associated with a delay in the kinetics and magnitude of NFATc nuclear localization. IL-4 production was restored genetically by retroviral reconstitution of Itk or biochemically by augmenting the calcium flux with ionomycin. In vivo, Itk-deficient mice were unable to establish functional Th2 cells. Development of protective Th1 cells was unimpeded. These data define a nonredundant role for Itk in modulating signals from the TCR/CD28 pathways that are specific for the establishment of stable IL-4 but not IFNgamma expression.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD28,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Interferon-gamma,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-2,
http://linkedlifedata.com/resource/pubmed/chemical/Interleukin-4,
http://linkedlifedata.com/resource/pubmed/chemical/Ionomycin,
http://linkedlifedata.com/resource/pubmed/chemical/Ionophores,
http://linkedlifedata.com/resource/pubmed/chemical/NFATC Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein-Tyrosine Kinases,
http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Antigen, T-Cell,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors,
http://linkedlifedata.com/resource/pubmed/chemical/emt protein-tyrosine kinase
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
1074-7613
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
11
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
399-409
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pubmed:dateRevised |
2009-11-19
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pubmed:meshHeading |
pubmed-meshheading:10549622-Animals,
pubmed-meshheading:10549622-Antigens, CD28,
pubmed-meshheading:10549622-Biological Transport,
pubmed-meshheading:10549622-Calcium Signaling,
pubmed-meshheading:10549622-Cell Differentiation,
pubmed-meshheading:10549622-Cell Division,
pubmed-meshheading:10549622-DNA-Binding Proteins,
pubmed-meshheading:10549622-Disease Progression,
pubmed-meshheading:10549622-Female,
pubmed-meshheading:10549622-Gene Expression Regulation,
pubmed-meshheading:10549622-Interferon-gamma,
pubmed-meshheading:10549622-Interleukin-2,
pubmed-meshheading:10549622-Interleukin-4,
pubmed-meshheading:10549622-Ionomycin,
pubmed-meshheading:10549622-Ionophores,
pubmed-meshheading:10549622-Leishmania major,
pubmed-meshheading:10549622-Leishmaniasis, Cutaneous,
pubmed-meshheading:10549622-Lymphocyte Activation,
pubmed-meshheading:10549622-Mice,
pubmed-meshheading:10549622-Mice, Inbred BALB C,
pubmed-meshheading:10549622-Mice, Inbred C57BL,
pubmed-meshheading:10549622-Mice, Knockout,
pubmed-meshheading:10549622-NFATC Transcription Factors,
pubmed-meshheading:10549622-Nuclear Proteins,
pubmed-meshheading:10549622-Protein-Tyrosine Kinases,
pubmed-meshheading:10549622-Receptors, Antigen, T-Cell,
pubmed-meshheading:10549622-Recombinant Fusion Proteins,
pubmed-meshheading:10549622-Specific Pathogen-Free Organisms,
pubmed-meshheading:10549622-Th2 Cells,
pubmed-meshheading:10549622-Transcription Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Impaired NFATc translocation and failure of Th2 development in Itk-deficient CD4+ T cells.
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pubmed:affiliation |
Department of Medicine, University of California San Francisco 94143, USA.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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