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rdf:type |
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lifeskim:mentions |
umls-concept:C0018340,
umls-concept:C0018353,
umls-concept:C0033684,
umls-concept:C0205314,
umls-concept:C0567416,
umls-concept:C0599851,
umls-concept:C0678640,
umls-concept:C0679622,
umls-concept:C0751984,
umls-concept:C1333684,
umls-concept:C1415311,
umls-concept:C1426113
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pubmed:issue |
1
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pubmed:dateCreated |
1999-12-17
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pubmed:abstractText |
Synaptic scaffolding molecule (S-SCAM) has six PDZ domains through which it interacts with N-methyl-d-aspartate receptors and neuroligin at synaptic junctions. We isolated here a novel S-SCAM-binding protein. This protein has one PDZ, one Ras association, one Ras GDP/GTP exchange protein (Ras GEP) domain, and one C-terminal consensus motif for binding to PDZ domains. We named it nRap GEP (neural Rap GEP). nRap GEP moreover has an incomplete cyclic AMP (cAMP)-binding (CAB) domain. The domain organization of nRap GEP is similar to that of Epac/cAMP-guanine nucleotide exchange factor (GEF) I, except that Epac/cAMP-GEFI has complete CAB and Ras GEP domains but lacks the other two domains and the C-terminal motif. nRap GEP showed GEP activity for Rap1 but did not bind cAMP. nRap GEP was specifically expressed in rat brain. Immunohistochemical analysis revealed that nRap GEP and S-SCAM were localized at synaptic areas of the cerebellum. These results suggest that nRap GEP is a novel neural Rap1-specific GEP which is associated with S-SCAM.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0006-291X
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pubmed:author |
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pubmed:copyrightInfo |
Copyright 1999 Academic Press.
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pubmed:issnType |
Print
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pubmed:volume |
265
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
38-44
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pubmed:dateRevised |
2011-11-17
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pubmed:meshHeading |
pubmed-meshheading:10548487-Adaptor Proteins, Signal Transducing,
pubmed-meshheading:10548487-Amino Acid Sequence,
pubmed-meshheading:10548487-Animals,
pubmed-meshheading:10548487-Binding Sites,
pubmed-meshheading:10548487-Brain,
pubmed-meshheading:10548487-Carrier Proteins,
pubmed-meshheading:10548487-Cloning, Molecular,
pubmed-meshheading:10548487-GTP-Binding Proteins,
pubmed-meshheading:10548487-Guanine Nucleotide Exchange Factors,
pubmed-meshheading:10548487-Guanylate Kinase,
pubmed-meshheading:10548487-Humans,
pubmed-meshheading:10548487-Kinetics,
pubmed-meshheading:10548487-Molecular Sequence Data,
pubmed-meshheading:10548487-Nerve Tissue Proteins,
pubmed-meshheading:10548487-Rats,
pubmed-meshheading:10548487-Recombinant Proteins,
pubmed-meshheading:10548487-Sequence Alignment,
pubmed-meshheading:10548487-Sequence Homology, Amino Acid,
pubmed-meshheading:10548487-Subcellular Fractions,
pubmed-meshheading:10548487-Synapses
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pubmed:year |
1999
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pubmed:articleTitle |
nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that interacts with synaptic scaffolding molecule (S-SCAM).
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pubmed:affiliation |
Department of Molecular Biology and Biochemistry, Osaka University Graduate School of Medicine/Faculty of Medicine, Suita, 565-0871, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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