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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
4
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pubmed:dateCreated |
1999-11-16
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pubmed:abstractText |
Allelic diversity at the Plasmodium vivax merozoite surface protein-3alpha (PvMsp-3alpha) locus was investigated using a combined polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) protocol. Symptomatic patient isolates from global geographic origins showed a high level of polymorphism at the nucleotide level. These samples were used to validate the sensitivity, specificity, and reproducibility of the PCR/RFLP method. It was then used to investigate PvMsp3alpha diversity in field samples from children living in a single village in a malaria-endemic region of Papua New Guinea, with the aim of assessing the usefulness of this locus as an epidemiologic marker of P. vivax infections. Eleven PvMsp-3alpha alleles were distinguishable in 16 samples with single infections, revealing extensive parasite polymorphism within this restricted area. Multiple infections were easily detected and accounted for 5 (23%) of 22 positive samples. Pairs of samples from individual children provided preliminary evidence for high turnover of P. vivax populations.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0002-9637
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
61
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
518-25
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pubmed:dateRevised |
2010-8-25
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pubmed:meshHeading |
pubmed-meshheading:10548283-Adolescent,
pubmed-meshheading:10548283-Animals,
pubmed-meshheading:10548283-Antigens, Protozoan,
pubmed-meshheading:10548283-Antigens, Surface,
pubmed-meshheading:10548283-Biological Markers,
pubmed-meshheading:10548283-Brazil,
pubmed-meshheading:10548283-Child,
pubmed-meshheading:10548283-Child, Preschool,
pubmed-meshheading:10548283-DNA, Protozoan,
pubmed-meshheading:10548283-DNA Primers,
pubmed-meshheading:10548283-El Salvador,
pubmed-meshheading:10548283-Electrophoresis, Agar Gel,
pubmed-meshheading:10548283-Genetic Variation,
pubmed-meshheading:10548283-Humans,
pubmed-meshheading:10548283-India,
pubmed-meshheading:10548283-Malaria, Vivax,
pubmed-meshheading:10548283-Papua New Guinea,
pubmed-meshheading:10548283-Parasitemia,
pubmed-meshheading:10548283-Plasmodium vivax,
pubmed-meshheading:10548283-Polymerase Chain Reaction,
pubmed-meshheading:10548283-Polymorphism, Restriction Fragment Length,
pubmed-meshheading:10548283-Protozoan Proteins,
pubmed-meshheading:10548283-Reproducibility of Results,
pubmed-meshheading:10548283-Sensitivity and Specificity,
pubmed-meshheading:10548283-Sri Lanka,
pubmed-meshheading:10548283-Sudan
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pubmed:year |
1999
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pubmed:articleTitle |
Polymorphism at the merozoite surface protein-3alpha locus of Plasmodium vivax: global and local diversity.
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pubmed:affiliation |
Wellcome Trust Centre for the Epidemiology of Infectious Disease, University of Oxford, United Kingdom.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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