Source:http://linkedlifedata.com/resource/pubmed/id/10545994
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Predicate | Object |
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
11
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pubmed:dateCreated |
1999-11-19
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pubmed:abstractText |
Thrombospondin forms a 'molecular bridge' between phagocytic and apoptotic cells through interaction with alphavbeta3/CD36. We report here that engagement of CD47, a newly described thrombospondin receptor, by immobilized monoclonal antibody against CD47 or by thrombospondin induced in all B-cell chronic lymphocytic leukemia clones the cytoplasmic features of apoptosis (cell shrinkage, decrease in mitochondrial transmembrane potential and phosphatidylserine externalization) without the nuclear features (chromatin condensation, appearance of single-stranded DNA, DNA fragmentation and cleavage of poly ADP-ribose polymerase). These cytoplasmic events of apoptosis were not prevented by the addition of caspase inhibitor z-VAD-fmk, or by the presence of survival factors (such as interleukin-4 and gamma interferon) or cell activation. Morphological studies confirmed the integrity of the nucleus and showed swelling of the mitochondria. This caspase-independent death pathway may be relevant to the development of alternate therapeutic strategies in chronic lymphocytic leukemia, which remains an incurable disease.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD47,
http://linkedlifedata.com/resource/pubmed/chemical/CD47 protein, human,
http://linkedlifedata.com/resource/pubmed/chemical/Carrier Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Caspases
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1078-8956
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pubmed:author | |
pubmed:issnType |
Print
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pubmed:volume |
5
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1277-84
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pubmed:dateRevised |
2007-11-15
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pubmed:meshHeading |
pubmed-meshheading:10545994-Adult,
pubmed-meshheading:10545994-Aged,
pubmed-meshheading:10545994-Antigens, CD,
pubmed-meshheading:10545994-Antigens, CD47,
pubmed-meshheading:10545994-Apoptosis,
pubmed-meshheading:10545994-Carrier Proteins,
pubmed-meshheading:10545994-Caspases,
pubmed-meshheading:10545994-Flow Cytometry,
pubmed-meshheading:10545994-Humans,
pubmed-meshheading:10545994-Leukemia, Lymphocytic, Chronic, B-Cell,
pubmed-meshheading:10545994-Middle Aged
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pubmed:year |
1999
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pubmed:articleTitle |
CD47 ligation induces caspase-independent cell death in chronic lymphocytic leukemia.
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pubmed:affiliation |
Laboratoire d'allergie, Centre de Recherche du Centre Hospitalier de l' Université de Montréal, Hôpital Notre-Dame, 1560 rue Sherbrooke Est, Université de Montréal H2L 4M1, Quebec, Canada.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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