Linked Life Data

10544286

Source:http://linkedlifedata.com/resource/pubmed/id/10544286

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
5
pubmed:dateCreated
2000-2-14
pubmed:abstractText
Fe-S cluster, the nonheme-iron cofactor essential for the activity of many proteins, is incorporated into its target protein by an unknown mechanism. In Escherichia coli, genes in the ORF1-ORF2-iscS-iscU-iscA-hscB-hsc A-fdx-ORF3 cluster (the isc gene cluster) should be involved in the assembly of the Fe-S cluster since its coexpression with the reporter ferredoxin (Fd) dramatically increases the production of holoFd [Nakamura, M., Saeki, K., and Takahashi, Y. (1999) J. Biochem. 126, 10-18]. In this study we addressed the functional roles of the proteins encoded by the isc gene cluster with respect to the assembly of Fe-S clusters in four reporter Fds. Plasmids were constructed in which eight ORFs in the isc gene cluster were individually inactivated either by truncating the coding region or by introducing an oligonucleotide linker containing stop codons. By coexpressing these plasmids with reporter Fds, we show the iscS, iscA, hscA, and fdx genes to be required for the assembly of the Fe-S clusters. When these genes were absent from the coexpression plasmid, no overproduction was achieved in any reporter Fds examined. The inactivation of ORF2 and hscB had a partial but appreciable effect on the production of some Fds. Deletion of ORF1 produced no difference from the coexpression with the intact isc gene cluster. We also examined coexpression using the fdx gene in the isc gene cluster as a reporter Fd and identified iscS, hscB, hscA, and ORF3 as being involved in the assembly of the [2Fe-2S] cluster in this protein. We propose a model in which the fdx gene product functions as an intermediate site for Fe-S cluster assembly.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0021-924X
pubmed:author
pubmed:issnType
Print
pubmed:volume
126
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
917-26
pubmed:dateRevised
2007-12-19
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Functional assignment of the ORF2-iscS-iscU-iscA-hscB-hscA-fdx-ORF3 gene cluster involved in the assembly of Fe-S clusters in Escherichia coli.
pubmed:affiliation
Department of Biology, Graduate School of Science, Osaka University, Toyonaka, Osaka, 560-0043, Japan. ytaka@bio.sci.osaka-u.ac.jp
pubmed:publicationType
Journal Article, Comparative Study, Research Support, Non-U.S. Gov't