Linked Life Data

10544136

Source:http://linkedlifedata.com/resource/pubmed/id/10544136

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-12-2
pubmed:abstractText
The heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) specifically binds to two transcription-regulatory elements, i.e., the leader and intergenic sequence, of the negative-strand (template-strand) RNA of mouse hepatitis virus (MHV) and may play a role in viral RNA transcription. Previous studies based on the defective-interfering RNAs of MHV suggested that these two RNA elements may interact with each other during transcription, although they do not have complementary sequences. In this study, we showed by an in vitro reconstitution assay that hnRNP A1 could mediate the formation of an RNP complex involving these two RNA elements. Both the RNA-binding domains and protein-interacting domain of hnRNP A1 contributed to the efficient formation of the RNP complex; however, the presence of the two RNA-binding domains alone, without the protein-interacting domain, also resulted in some RNP formation. Omission of hnRNP A1 in the reconstitution reaction abolished the RNP formation, and mutations of the IG sequences significantly inhibited the RNP formation. These findings suggest that the two cis-acting transcription-regulatory sequences of MHV RNA can interact with each other through the formation of an RNP complex involving a cellular protein hnRNP A1. This RNP complex may participate in MHV RNA transcription.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0042-6822
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:day
10
pubmed:volume
264
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
115-24
pubmed:dateRevised
2007-11-14
pubmed:meshHeading
pubmed-meshheading:10544136-Animals, pubmed-meshheading:10544136-Cloning, Molecular, pubmed-meshheading:10544136-DNA Primers, pubmed-meshheading:10544136-Heterogeneous-Nuclear Ribonucleoprotein Group A-B, pubmed-meshheading:10544136-Heterogeneous-Nuclear Ribonucleoproteins, pubmed-meshheading:10544136-Mice, pubmed-meshheading:10544136-Murine hepatitis virus, pubmed-meshheading:10544136-Polymerase Chain Reaction, pubmed-meshheading:10544136-Protein Biosynthesis, pubmed-meshheading:10544136-RNA, Viral, pubmed-meshheading:10544136-Rabbits, pubmed-meshheading:10544136-Recombinant Fusion Proteins, pubmed-meshheading:10544136-Regulatory Sequences, Nucleic Acid, pubmed-meshheading:10544136-Reticulocytes, pubmed-meshheading:10544136-Ribonucleoproteins, pubmed-meshheading:10544136-Transcription, Genetic
pubmed:year
1999
pubmed:articleTitle
Formation of a ribonucleoprotein complex of mouse hepatitis virus involving heterogeneous nuclear ribonucleoprotein A1 and transcription-regulatory elements of viral RNA.
pubmed:affiliation
Department of Neurology, University of Southern California School of Medicine, Los Angeles, California 90033, USA.
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't