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rdf:type |
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lifeskim:mentions |
umls-concept:C0012854,
umls-concept:C0025663,
umls-concept:C0180860,
umls-concept:C0185125,
umls-concept:C0204727,
umls-concept:C0205409,
umls-concept:C0580794,
umls-concept:C0581406,
umls-concept:C0596901,
umls-concept:C1511790,
umls-concept:C1522664,
umls-concept:C1546637,
umls-concept:C1550638,
umls-concept:C1704449,
umls-concept:C1704684
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pubmed:issue |
11
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pubmed:dateCreated |
1999-12-13
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pubmed:abstractText |
Verotoxigenic Escherichia coli (VTEC) strains were isolated from food and animal fecal samples by using PCR to screen for the presence of VTEC after broth enrichment and then filtering VTEC-positive cultures through hydrophobic-grid membrane filters (HGMFs) which were incubated on MacConkey agar. The filters were probed with a digoxigenin-labeled PCR product generated by amplification of a conserved verotoxin gene sequence. Replication of the growth on filters allowed probe-positive colonies to be picked. When ground beef samples were inoculated with VTEC strains, 100% of the strains were recovered, and the detection limit was 0.1 CFU per g. Similar results were obtained with seven types of artificially contaminated vegetables. A survey of 32 packages of vegetables and 23 samples of apple cider obtained at the retail level did not reveal the presence of VTEC. However, the intestinal fecal contents of a moose, 1 of 35 wild mammals and birds examined, contained E. coli O157:H7. The DNA hybridization-HGMF method was also used in a prevalence survey of 327 raw and 744 ready-to-eat products; VTEC strains were recovered from 4.9% of the raw products and 0.7% of the ready-to-eat products. No serotype O157:H7 strains were detected. This method is particularly suited for surveys in which low numbers of VTEC-positive samples are expected and isolates are required.
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pubmed:commentsCorrections |
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-1513344,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-1514799,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-1569356,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-1901711,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-2182671,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-2187410,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-2200697,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-2230244,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-2268161,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-2649386,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-3060018,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-338490,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-3524458,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-7781732,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-8161171,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-8406845,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-8423084,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-8625998,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-8660186,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-8760944,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-8874070,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-8935562,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-9192033,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-9361444,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-9665978,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-9797257,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-9841830,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-9856639,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-9866741,
http://linkedlifedata.com/resource/pubmed/commentcorrection/10543785-9927001
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0099-2240
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
65
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4775-80
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pubmed:dateRevised |
2009-11-18
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pubmed:meshHeading |
pubmed-meshheading:10543785-Animals,
pubmed-meshheading:10543785-Bacterial Toxins,
pubmed-meshheading:10543785-Beverages,
pubmed-meshheading:10543785-Birds,
pubmed-meshheading:10543785-Deer,
pubmed-meshheading:10543785-Escherichia coli O157,
pubmed-meshheading:10543785-Feces,
pubmed-meshheading:10543785-Food Microbiology,
pubmed-meshheading:10543785-Fruit,
pubmed-meshheading:10543785-Mammals,
pubmed-meshheading:10543785-Meat,
pubmed-meshheading:10543785-Polymerase Chain Reaction,
pubmed-meshheading:10543785-Shiga Toxin 1,
pubmed-meshheading:10543785-Vegetables
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pubmed:year |
1999
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pubmed:articleTitle |
Application of a DNA hybridization-hydrophobic-grid membrane filter method for detection and isolation of verotoxigenic escherichia coli.
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pubmed:affiliation |
Bureau of Microbial Hazards, Food Directorate, Health Protection Branch, Health Canada, Ottawa, Ontario K1A 0L2. ewen_todd@hc-sc.gc.ca
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pubmed:publicationType |
Journal Article
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