Linked Life Data

10542371

Source:http://linkedlifedata.com/resource/pubmed/id/10542371

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
1999-12-21
pubmed:abstractText
The relaxin-like factor (RLF) is a novel member of the insulin-IGF-relaxin family of growth factors and hormones, and its mRNA is expressed very specifically in the Leydig cells of the testis and in the theca and luteal cells of the ovary. Here we report the cloning of the RLF gene and cDNA from the rat. The 0.8kb mRNA is produced from a small gene comprising two exons situated less than 1 kb downstream of the gene for the signalling factor JAK3. Northern hybridization confirms high RLF mRNA expression in the adult rat testis, and low expression in the ovary, but in no other tissues examined. Northern analysis of fetal and neonatal gonadal tissues showed that RLF mRNA is highly upregulated in the testes of day 19 embryos, but not in later neonatal stages, nor in any ovarian tissue from this period. This would indicate that RLF is a marker for the mature fetal as well as the adult-type Leydig cell, but is not expressed in premature, precursor, or dedifferentiated Leydig cells of either cell type. Finally, RNA was analysed from the testes of rats which had been treated with ethylene dimethane sulfonate (EDS), an alkylating agent that specifically destroys rat Leydig cells. RLF mRNA was absent from the acutely treated testes, but became detectable between 15 and 20 days post-treatment, concomitant with the repopulation of the testes by new Leydig cells. Continuous testosterone substitution of EDS-treated rats suppressed the production of gonadotropins, and LH-dependent Leydig cell differentiation, with the result that RLF mRNA remained undetectable throughout the study period. In conclusion, RLF is a very specific marker for the mature Leydig cell phenotype in both the adult-type and fetal Leydig cell populations of the rat testis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
1040-452X
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
54
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
319-25
pubmed:dateRevised
2011-11-17
pubmed:meshHeading
pubmed-meshheading:10542371-Amino Acid Sequence, pubmed-meshheading:10542371-Animals, pubmed-meshheading:10542371-Base Sequence, pubmed-meshheading:10542371-Blotting, Northern, pubmed-meshheading:10542371-Cloning, Molecular, pubmed-meshheading:10542371-DNA, Complementary, pubmed-meshheading:10542371-Female, pubmed-meshheading:10542371-Gene Expression Regulation, Developmental, pubmed-meshheading:10542371-Insulin, pubmed-meshheading:10542371-Janus Kinase 3, pubmed-meshheading:10542371-Leydig Cells, pubmed-meshheading:10542371-Male, pubmed-meshheading:10542371-Mice, pubmed-meshheading:10542371-Molecular Sequence Data, pubmed-meshheading:10542371-Polymerase Chain Reaction, pubmed-meshheading:10542371-Protein-Tyrosine Kinases, pubmed-meshheading:10542371-Proteins, pubmed-meshheading:10542371-Rats, pubmed-meshheading:10542371-Rats, Sprague-Dawley, pubmed-meshheading:10542371-Reading Frames, pubmed-meshheading:10542371-Sequence Analysis, DNA, pubmed-meshheading:10542371-Sequence Homology, Amino Acid, pubmed-meshheading:10542371-Testis
pubmed:year
1999
pubmed:articleTitle
Structure and expression of the rat relaxin-like factor (RLF) gene.
pubmed:affiliation
Institute for Hormone and Fertility Research, University of Hamburg, Hamburg, Germany.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't