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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
5
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pubmed:dateCreated |
1999-11-19
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pubmed:abstractText |
Preproenkephalin (PPNK) mRNA expression has been detected in many cells of the immune system, including monocytes and lymphocytes. In the present paper, the expression of PPNK mRNA in purified CD4+ Th1 and Th2 lymphocyte subpopulations is investigated and correlated with the presence of the opioid neuropeptides leu- and met-enkephalin. We found that PPNK mRNA and met-enkephalin were present at higher levels in the Th2 cultures compared with the Th1 cultures. Lymphocytes from PPNK-deficient mice were then used to look at the role of PPNK in Th2 lymphocyte differentiation. Lymphocytes from these mice could be driven into a Th2 phenotype, suggesting that cultures containing IL-4 do not require PPNK for Th2 differentiation.
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pubmed:grant |
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Oct
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pubmed:issn |
0818-9641
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
77
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
385-90
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pubmed:dateRevised |
2009-9-29
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pubmed:meshHeading |
pubmed-meshheading:10540203-Animals,
pubmed-meshheading:10540203-Cell Differentiation,
pubmed-meshheading:10540203-Cells, Cultured,
pubmed-meshheading:10540203-Enkephalin, Leucine,
pubmed-meshheading:10540203-Enkephalin, Methionine,
pubmed-meshheading:10540203-Enkephalins,
pubmed-meshheading:10540203-Female,
pubmed-meshheading:10540203-Flow Cytometry,
pubmed-meshheading:10540203-Interleukin-4,
pubmed-meshheading:10540203-Male,
pubmed-meshheading:10540203-Mice,
pubmed-meshheading:10540203-Mice, Inbred C57BL,
pubmed-meshheading:10540203-Mice, Knockout,
pubmed-meshheading:10540203-Polymerase Chain Reaction,
pubmed-meshheading:10540203-Protein Precursors,
pubmed-meshheading:10540203-RNA, Messenger,
pubmed-meshheading:10540203-Th1 Cells,
pubmed-meshheading:10540203-Th2 Cells
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pubmed:year |
1999
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pubmed:articleTitle |
Preproenkephalin is a Th2 cytokine but is not required for Th2 differentiation in vitro.
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pubmed:affiliation |
Malaghan Institute of Medical Research, Wellington School of Medicine, Wellington, New Zealand.mimrsh@wnmeds.ac.nz
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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