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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
22
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pubmed:dateCreated |
1999-12-28
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pubmed:abstractText |
Promoters recognized by the RNA-polymerase with the alternative sigma factor sigma(54) (Esigma54) are unique in having conserved positions around -24 and -12 nucleotides upstream from the transcriptional start site, instead of the typical -35 and -10 boxes. Here we compile 186 -24/-12 promoter sequences reported in the literature and generate an updated and extended consensus sequence. The use of the extended consensus increases the probability of identifying genuine -24/-12 promoters. The effect of several reported mutations at the -24/-12 elements on RNA-polymerase binding and promoter strength is discussed in the light of the updated consensus.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
1362-4962
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pubmed:author |
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pubmed:issnType |
Electronic
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pubmed:day |
15
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pubmed:volume |
27
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
4305-13
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10536136-Bacteria,
pubmed-meshheading:10536136-Base Sequence,
pubmed-meshheading:10536136-Consensus Sequence,
pubmed-meshheading:10536136-Conserved Sequence,
pubmed-meshheading:10536136-DNA, Bacterial,
pubmed-meshheading:10536136-DNA-Binding Proteins,
pubmed-meshheading:10536136-DNA-Directed RNA Polymerases,
pubmed-meshheading:10536136-Databases, Factual,
pubmed-meshheading:10536136-Molecular Sequence Data,
pubmed-meshheading:10536136-Mutation,
pubmed-meshheading:10536136-Promoter Regions, Genetic,
pubmed-meshheading:10536136-RNA Polymerase Sigma 54,
pubmed-meshheading:10536136-Sequence Homology, Nucleic Acid,
pubmed-meshheading:10536136-Sigma Factor,
pubmed-meshheading:10536136-Transcription, Genetic
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pubmed:year |
1999
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pubmed:articleTitle |
Compilation and analysis of sigma(54)-dependent promoter sequences.
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pubmed:affiliation |
Departamento de Reconocimiento Molecular y Bioestructura, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62271, México.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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