Source:http://linkedlifedata.com/resource/pubmed/id/10536032
Switch to
| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
11
|
| pubmed:dateCreated |
2000-1-19
|
| pubmed:abstractText |
Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A(4)(GS I-A(4)), which is cytotoxic to the human colon cancer cell lines, is one of two lectin families derived from its seed extract. It contains only a homo-oligomer of subunit A, and is most specific for GalNAcalpha1-->. In order to elucidate the GS I-A(4)-glycoconjugate interactions in greater detail, the combining site of this lectin was further characterized by enzyme linked lectino-sorbent assay (ELLSA) and by inhibition of lectin-glycoprotein interactions. This study has demonstrated that the Tn-containing glycoproteins tested, consisting of mammalian salivary glycoproteins (armadillo, asialo-hamster sublingual, asialo-ovine, -bovine, and -porcine submandibular), are bound strongly by GS I-A(4.)Among monovalent inhibitors so far tested, p-NO2-phenylalphaGalNAc is the most potent, suggesting that hydrophobic forces are important in the interaction of this lectin. GS I-A(4)is able to accommodate the monosaccharide GalNAc at the nonreducing end of oligosaccharides. This suggests that the combining site of the lectin is a shallow cavity. Among oligosaccharides and monosaccharides tested as inhibitors of the binding of GS I-A(4), the hierarchy of potencies are: GalNAcalpha1-->3GalNAcbeta1-->3Galalpha1-->4Galbeta 1-->4Glc (Forssman pentasaccharide) > GalNAcalpha1-->3(LFucalpha1-->2)Gal (blood group A)()> GalNAc > Galalpha1-->4Gal > Galalpha1-->3Gal (blood group B-like)> Gal.
|
| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Blood Group Antigens,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Griffonia simplicifolia lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Ligands,
http://linkedlifedata.com/resource/pubmed/chemical/Oligosaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Plant Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Salivary Proteins and Peptides
|
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0959-6658
|
| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
9
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
1161-70
|
| pubmed:dateRevised |
2008-11-21
|
| pubmed:meshHeading |
pubmed-meshheading:10536032-Animals,
pubmed-meshheading:10536032-Armadillos,
pubmed-meshheading:10536032-Blood Group Antigens,
pubmed-meshheading:10536032-Carbohydrate Sequence,
pubmed-meshheading:10536032-Cricetinae,
pubmed-meshheading:10536032-Echinococcosis,
pubmed-meshheading:10536032-Female,
pubmed-meshheading:10536032-Glycoproteins,
pubmed-meshheading:10536032-Humans,
pubmed-meshheading:10536032-Lectins,
pubmed-meshheading:10536032-Ligands,
pubmed-meshheading:10536032-Molecular Sequence Data,
pubmed-meshheading:10536032-Oligosaccharides,
pubmed-meshheading:10536032-Ovarian Cysts,
pubmed-meshheading:10536032-Plant Lectins,
pubmed-meshheading:10536032-Protein Binding,
pubmed-meshheading:10536032-Salivary Proteins and Peptides,
pubmed-meshheading:10536032-Sheep,
pubmed-meshheading:10536032-Sheep Diseases,
pubmed-meshheading:10536032-Swine
|
| pubmed:year |
1999
|
| pubmed:articleTitle |
Further characterization of the combining sites of Bandeiraea (Griffonia) simplicifolia lectin-I, isolectin A(4).
|
| pubmed:affiliation |
Glyco-Immunochemistry Research Lab, Institute of Molecular and Cellular Biology, Chang-Gung University, Taiwan.
|
| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, U.S. Gov't, Non-P.H.S.,
Research Support, Non-U.S. Gov't
|