Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
4
pubmed:dateCreated
2000-1-12
pubmed:abstractText
Seroepidemiology studies of herpes simplex virus type 2 (HSV-2) infections have been difficult to carry out because antibodies to HSV type 1 (HSV-1) show an extensive cross-reactivity with HSV-2 antigens. Many kits available currently are not entirely type specific for serodiagnosis of HSV-2 infections and therefore do not allow reliable discrimination of past exposure to these closely related alphaherpes viruses. Attempts to develop type-specific antigens have focused on the envelope glycoproteins, particularly glycoprotein G (gG). A cross-sectional study was carried out to examine the seroprevalence of antibodies to HSV-2 among healthy university students, using different methods: a whole cell lysate enzyme-linked immunosorbent assay (ELISA), two different ELISAs, and a newly developed immunoblot assay, the last three based on recombinant gG2. HSV-2 prevalence was 24 times higher with the whole cell lysate ELISA (31%; 95% confidence interval [CI]: 27-35%) than the ELISAs and the immunoblot assay based on recombinant gG2 (1.3%; 95% CI: 0.1-2.5%), thus showing the inaccuracy of commercial tests based on whole-antigen preparations for epidemiological studies. Laboratories should be cautious and ensure that commercial tests for HSV typing are based on type-specific glycoproteins.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Dec
pubmed:issn
0146-6615
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Wiley-Liss, Inc.
pubmed:issnType
Print
pubmed:volume
59
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
502-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10534733-Adolescent, pubmed-meshheading:10534733-Adult, pubmed-meshheading:10534733-Antibodies, Viral, pubmed-meshheading:10534733-Antigens, Viral, pubmed-meshheading:10534733-Blotting, Western, pubmed-meshheading:10534733-Cross-Sectional Studies, pubmed-meshheading:10534733-Enzyme-Linked Immunosorbent Assay, pubmed-meshheading:10534733-Female, pubmed-meshheading:10534733-Herpes Genitalis, pubmed-meshheading:10534733-Herpesvirus 1, Human, pubmed-meshheading:10534733-Herpesvirus 2, Human, pubmed-meshheading:10534733-Humans, pubmed-meshheading:10534733-Immunoblotting, pubmed-meshheading:10534733-Immunoglobulin G, pubmed-meshheading:10534733-Male, pubmed-meshheading:10534733-Recombinant Proteins, pubmed-meshheading:10534733-Seroepidemiologic Studies, pubmed-meshheading:10534733-Viral Envelope Proteins
pubmed:year
1999
pubmed:articleTitle
Whole cell lysate enzyme immunoassays vs. recombinant glycoprotein G2-based immunoassays for HSV-2 seroprevalence studies.
pubmed:affiliation
Medical Department, SmithKline Beecham Pharmaceuticals, Madrid, Spain.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't