Source:http://linkedlifedata.com/resource/pubmed/id/10534733
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rdf:type | |
lifeskim:mentions | |
pubmed:issue |
4
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pubmed:dateCreated |
2000-1-12
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pubmed:abstractText |
Seroepidemiology studies of herpes simplex virus type 2 (HSV-2) infections have been difficult to carry out because antibodies to HSV type 1 (HSV-1) show an extensive cross-reactivity with HSV-2 antigens. Many kits available currently are not entirely type specific for serodiagnosis of HSV-2 infections and therefore do not allow reliable discrimination of past exposure to these closely related alphaherpes viruses. Attempts to develop type-specific antigens have focused on the envelope glycoproteins, particularly glycoprotein G (gG). A cross-sectional study was carried out to examine the seroprevalence of antibodies to HSV-2 among healthy university students, using different methods: a whole cell lysate enzyme-linked immunosorbent assay (ELISA), two different ELISAs, and a newly developed immunoblot assay, the last three based on recombinant gG2. HSV-2 prevalence was 24 times higher with the whole cell lysate ELISA (31%; 95% confidence interval [CI]: 27-35%) than the ELISAs and the immunoblot assay based on recombinant gG2 (1.3%; 95% CI: 0.1-2.5%), thus showing the inaccuracy of commercial tests based on whole-antigen preparations for epidemiological studies. Laboratories should be cautious and ensure that commercial tests for HSV typing are based on type-specific glycoproteins.
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pubmed:language |
eng
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pubmed:journal | |
pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antibodies, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Viral,
http://linkedlifedata.com/resource/pubmed/chemical/Immunoglobulin G,
http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Viral Envelope Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/glycoprotein G, herpes simplex...
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pubmed:status |
MEDLINE
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pubmed:month |
Dec
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pubmed:issn |
0146-6615
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pubmed:author | |
pubmed:copyrightInfo |
Copyright 1999 Wiley-Liss, Inc.
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pubmed:issnType |
Print
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pubmed:volume |
59
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
502-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10534733-Adolescent,
pubmed-meshheading:10534733-Adult,
pubmed-meshheading:10534733-Antibodies, Viral,
pubmed-meshheading:10534733-Antigens, Viral,
pubmed-meshheading:10534733-Blotting, Western,
pubmed-meshheading:10534733-Cross-Sectional Studies,
pubmed-meshheading:10534733-Enzyme-Linked Immunosorbent Assay,
pubmed-meshheading:10534733-Female,
pubmed-meshheading:10534733-Herpes Genitalis,
pubmed-meshheading:10534733-Herpesvirus 1, Human,
pubmed-meshheading:10534733-Herpesvirus 2, Human,
pubmed-meshheading:10534733-Humans,
pubmed-meshheading:10534733-Immunoblotting,
pubmed-meshheading:10534733-Immunoglobulin G,
pubmed-meshheading:10534733-Male,
pubmed-meshheading:10534733-Recombinant Proteins,
pubmed-meshheading:10534733-Seroepidemiologic Studies,
pubmed-meshheading:10534733-Viral Envelope Proteins
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pubmed:year |
1999
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pubmed:articleTitle |
Whole cell lysate enzyme immunoassays vs. recombinant glycoprotein G2-based immunoassays for HSV-2 seroprevalence studies.
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pubmed:affiliation |
Medical Department, SmithKline Beecham Pharmaceuticals, Madrid, Spain.
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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