Source:http://linkedlifedata.com/resource/pubmed/id/10534340
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
5
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| pubmed:dateCreated |
1999-11-24
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| pubmed:abstractText |
The insulin-like growth factor binding protein-1 (IGFBP-1) gene is highly expressed in fetal, perinatal, and regenerating liver. Up-regulation is transcriptionally mediated in regenerating liver and occurs in the first few minutes to hours after partial hepatectomy. In transgenic mice a 970-bp region from -776 to +151 of the IGFBP-1 promoter was sufficient for tissue-specific and induced expression of the gene in fetal and hepatectomized livers. However weak and/or poorly regulated expression in some transgenic lines suggested the existence of other regulatory regions. Here, genomic clones containing large regions 5' of the mouse IGFBP-1 gene sequence were isolated, subcloned, and sequenced. Deoxyribonuclease I (DNaseI) hypersensitivity analyses identified clusters of tissue-specific nuclease-sensitive sites in the promoter region, -100 to -300, -2,300, -3,100, and -5,000 along with other weak sites. After partial hepatectomy, enhanced sensitivity and/or novel sites were detected in the -100/-300, -5,000, and -3,100 regions, the promoter region remaining the most hypersensitive. A subset of these sites was present in fetal and perinatal livers. Novel tissue-specific sites that interacted with C/EBP and hepatic nuclear factor 3 (HNF3) transcription factors were identified in the -3,100 region. A hepatectomy-induced DNA binding complex containing the transcription factor USF1 was identified within the -100 to -300 region of the promoter. These results suggested that a complex array of tissue-specific and hepatic proliferation-induced transcription factors combine to regulate both the proximal promoter and more distal regulatory elements of the IGFBP-1 gene.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
IM
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| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Nov
|
| pubmed:issn |
0270-9139
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:volume |
30
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
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| pubmed:pagination |
1187-97
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10534340-Animals,
pubmed-meshheading:10534340-Base Sequence,
pubmed-meshheading:10534340-Carcinoma, Hepatocellular,
pubmed-meshheading:10534340-Cloning, Molecular,
pubmed-meshheading:10534340-Deoxyribonuclease I,
pubmed-meshheading:10534340-Fetus,
pubmed-meshheading:10534340-Gene Expression Regulation, Developmental,
pubmed-meshheading:10534340-Hepatectomy,
pubmed-meshheading:10534340-Humans,
pubmed-meshheading:10534340-Insulin-Like Growth Factor Binding Protein 1,
pubmed-meshheading:10534340-Kidney,
pubmed-meshheading:10534340-Liver,
pubmed-meshheading:10534340-Liver Neoplasms,
pubmed-meshheading:10534340-Liver Regeneration,
pubmed-meshheading:10534340-Mice,
pubmed-meshheading:10534340-Mice, Transgenic,
pubmed-meshheading:10534340-Molecular Sequence Data,
pubmed-meshheading:10534340-Promoter Regions, Genetic,
pubmed-meshheading:10534340-Recombinant Proteins,
pubmed-meshheading:10534340-Regulatory Sequences, Nucleic Acid,
pubmed-meshheading:10534340-Restriction Mapping,
pubmed-meshheading:10534340-Sequence Alignment,
pubmed-meshheading:10534340-Sequence Homology, Nucleic Acid,
pubmed-meshheading:10534340-Spleen,
pubmed-meshheading:10534340-Substrate Specificity,
pubmed-meshheading:10534340-Transfection,
pubmed-meshheading:10534340-Tumor Cells, Cultured
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| pubmed:year |
1999
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| pubmed:articleTitle |
Liver-specific and proliferation-induced deoxyribonuclease I hypersensitive sites in the mouse insulin-like growth factor binding protein-1 gene.
|
| pubmed:affiliation |
Department of Genetics, Division of Gastroenterology, University of Pennsylvania School of Medicine, Philadelphia, PA 19104, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|