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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6
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pubmed:dateCreated |
1979-9-17
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pubmed:abstractText |
Microsomes isolated from the fibroin region of the Bombyx mori silkgland synthesize in the cell-free system a glycin rich polypeptide or polypeptides presumably representing fibroin precursors. Besides microsomes the system requires ATP and ATP-generating system, GTP, soluble protein fraction and tRNA, glycine incorporation is inhibited by puromycin and cycloheximide. It is shown that the synthesis of a polypeptide with high Gly/Lys ratio requires soluble protein fraction isolated from the silk gland at the end of the instar V. When the soluble protein fraction from the larvoe at the early instar V is used the Gly/Lys ratio in the product is markedly lower. These results permit to suggest that fibroin synthesis may be regulated at the level of tRNA aminoacylation.
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pubmed:language |
rus
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:issn |
0026-8984
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:volume |
10
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
1355-9
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:1053090-Adenosine Triphosphate,
pubmed-meshheading:1053090-Animals,
pubmed-meshheading:1053090-Bombyx,
pubmed-meshheading:1053090-Cell-Free System,
pubmed-meshheading:1053090-Cycloheximide,
pubmed-meshheading:1053090-Fibroins,
pubmed-meshheading:1053090-Guanosine Triphosphate,
pubmed-meshheading:1053090-Kinetics,
pubmed-meshheading:1053090-Microsomes,
pubmed-meshheading:1053090-Protein Biosynthesis,
pubmed-meshheading:1053090-Puromycin,
pubmed-meshheading:1053090-RNA, Transfer
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pubmed:articleTitle |
[Properties of the cell-free protein synthesizing system from the fibroin region of the Bombyx mori silkgland].
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pubmed:publicationType |
Journal Article,
English Abstract
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