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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
9
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pubmed:dateCreated |
1999-11-19
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pubmed:databankReference |
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pubmed:abstractText |
C-type lectins serve multiple functions through recognizing carbohydrate chains. Here we report a novel C-type lectin, macrophage-inducible C-type lectin (Mincle), as a downstream target of NF-IL6 in macrophages. NF-IL6 belongs to the CCAAT/enhancer binding protein (C/EBP) of transcription factors and plays a crucial role in activated macrophages. However, what particular genes are regulated by NF-IL6 has been poorly defined in macrophages. Identification of downstream targets is required to elucidate the function of NF-IL6 in more detail. To identify downstream genes of NF-IL6, we screened a subtraction library constructed from wild-type and NF-IL6-deficient peritoneal macrophages and isolated Mincle that exhibits the highest homology to the members of group II C-type lectins. Mincle mRNA expression was strongly induced in response to several inflammatory stimuli, such as LPS, TNF-alpha, IL-6, and IFN-gamma in wild-type macrophages. In contrast, NF-IL6-deficient macrophages displayed a much lower level of Mincle mRNA induction following treatment with these inflammatory reagents. The mouse Mincle proximal promoter region contains an indispensable NF-IL6 binding element, demonstrating that Mincle is a direct target of NF-IL6. The Mincle gene locus was mapped at 0.6 centiMorgans proximal to CD4 on mouse chromosome 6.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
AIM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/CCAAT-Enhancer-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Clecsf8 protein, mouse,
http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins,
http://linkedlifedata.com/resource/pubmed/chemical/Lectins, C-Type,
http://linkedlifedata.com/resource/pubmed/chemical/Lipopolysaccharides,
http://linkedlifedata.com/resource/pubmed/chemical/Membrane Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Nuclear Proteins,
http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors
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pubmed:status |
MEDLINE
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pubmed:month |
Nov
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pubmed:issn |
0022-1767
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
1
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pubmed:volume |
163
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
5039-48
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pubmed:dateRevised |
2008-11-21
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pubmed:meshHeading |
pubmed-meshheading:10528209-Animals,
pubmed-meshheading:10528209-CCAAT-Enhancer-Binding Proteins,
pubmed-meshheading:10528209-Chromosome Mapping,
pubmed-meshheading:10528209-Cloning, Molecular,
pubmed-meshheading:10528209-DNA-Binding Proteins,
pubmed-meshheading:10528209-Female,
pubmed-meshheading:10528209-Gene Expression Regulation,
pubmed-meshheading:10528209-Lectins,
pubmed-meshheading:10528209-Lectins, C-Type,
pubmed-meshheading:10528209-Lipopolysaccharides,
pubmed-meshheading:10528209-Macrophages, Peritoneal,
pubmed-meshheading:10528209-Male,
pubmed-meshheading:10528209-Membrane Proteins,
pubmed-meshheading:10528209-Mice,
pubmed-meshheading:10528209-Mice, Inbred C57BL,
pubmed-meshheading:10528209-Mice, Knockout,
pubmed-meshheading:10528209-Nuclear Proteins,
pubmed-meshheading:10528209-Promoter Regions, Genetic,
pubmed-meshheading:10528209-Protein Binding,
pubmed-meshheading:10528209-Transcription, Genetic,
pubmed-meshheading:10528209-Transcription Factors
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pubmed:year |
1999
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pubmed:articleTitle |
A novel LPS-inducible C-type lectin is a transcriptional target of NF-IL6 in macrophages.
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pubmed:affiliation |
Department of Host Defense, Research Institute for Microbial Diseases, Osaka University, Japan.
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pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.,
Research Support, Non-U.S. Gov't
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