Source:http://linkedlifedata.com/resource/pubmed/id/10528188
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
1999-11-19
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| pubmed:abstractText |
Langerhans cells (LC) are CD1a+E-cadherin (E-cad)+Birbeck granule+ but CD11b-CD36-factor XIIIa (FXIIIa)- members of the dendritic cell (DC) family. Evidence holds that LC originate from CD1a+CD14- rather than CD14+CD1a- progenitors, both of which arise from GM-CSF/TNF-alpha-stimulated CD34+ stem cells. The CD14+CD1a- progenitors, on the other hand, can give rise to a separate DC type characterized by its CD1a+CD11b+CD36+FXIIIa+E-cad-BG- phenotype (non-LC DC). Although GM-CSF/TNF-alpha are important for both LC and non-LC DC differentiation, TGF-beta 1 is thought to preferentially promote LC development in vitro and in vivo. However, the hemopoietic biology of this process and the nature of TGF-beta 1-responsive LC precursors (LCp) are not well understood. Here we show that CD14+ precursors in the presence, but not in the absence, of TGF-beta 1 give rise to a progeny that fulfills all major criteria of LC. In contrast, LC development from CD1a+ progenitors was TGF-beta 1 independent. Further studies revealed that CD14+ precursors contain a CD11b+ and a CD11b- subpopulation. When either subset was stimulated with GM-CSF/TNF-alpha and TGF-beta 1, only CD14+CD11b- cells differentiated into LC. The CD11b+ cells, on the other hand, acquired non-LC DC features only. The higher doubling rates of cells entering the CD14+ LCp rather than the CD1a+ LCp pathway add to the importance of TGF-beta 1 for LC development. Because CD14+CD11b- precursors are multipotent cells that can enter LC or macrophage differentiation, it is suggested that these cells, if present at the tissue level, endow a given organ with the property to generate diverse cell types in response to the local cytokine milieu.
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| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
|
| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD1,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD14,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, CD34,
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Surface,
http://linkedlifedata.com/resource/pubmed/chemical/Transforming Growth Factor beta
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
|
| pubmed:day |
1
|
| pubmed:volume |
163
|
| pubmed:owner |
NLM
|
| pubmed:authorsComplete |
Y
|
| pubmed:pagination |
4869-77
|
| pubmed:dateRevised |
2006-11-15
|
| pubmed:meshHeading |
pubmed-meshheading:10528188-Adult,
pubmed-meshheading:10528188-Antigens, CD1,
pubmed-meshheading:10528188-Antigens, CD14,
pubmed-meshheading:10528188-Antigens, CD34,
pubmed-meshheading:10528188-Antigens, Surface,
pubmed-meshheading:10528188-Cell Differentiation,
pubmed-meshheading:10528188-Cell Division,
pubmed-meshheading:10528188-Cells, Cultured,
pubmed-meshheading:10528188-Cytoplasmic Granules,
pubmed-meshheading:10528188-Dendritic Cells,
pubmed-meshheading:10528188-Fetal Blood,
pubmed-meshheading:10528188-Hematopoietic Stem Cells,
pubmed-meshheading:10528188-Humans,
pubmed-meshheading:10528188-Interphase,
pubmed-meshheading:10528188-Langerhans Cells,
pubmed-meshheading:10528188-Lymphocyte Activation,
pubmed-meshheading:10528188-Macrophages,
pubmed-meshheading:10528188-T-Lymphocytes,
pubmed-meshheading:10528188-Transforming Growth Factor beta
|
| pubmed:year |
1999
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| pubmed:articleTitle |
CD34+ cell-derived CD14+ precursor cells develop into Langerhans cells in a TGF-beta 1-dependent manner.
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| pubmed:affiliation |
Division of Immunology, Allergy, and Infectious Diseases, Department of Dermatology, University of Vienna Medical School, Austria.
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| pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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