Source:http://linkedlifedata.com/resource/pubmed/id/10528179
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
9
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| pubmed:dateCreated |
1999-11-19
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| pubmed:abstractText |
The SM6C10 IgM autoantibody recognizes a surface determinant, 6C10, that is highly expressed on all immature thymocytes. In contrast, its expression on peripheral T cells appears developmentally regulated, i.e., absent from most naive T cells in spleen of neonatal mice, but expressed on 40-80% of naive CD4+ T cells in adult. In this paper, we demonstrate that SM6C10 recognizes a carbohydrate epitope on the Thy-1 glycoprotein using immunoprecipitation analysis, by binding to affinity-purified Thy-1 in an ELISA, and by sensitivity to N-glycosidase-F treatment. Retroviral Thy-1 gene transduction experiments into Thy-1- variant T cell lines and a pro-B cell line provide evidence that 6C10 glycosylated Thy-1 expression is not restricted to T cells but depends on the recipient cell. Therefore, differences in 6C10 levels among Thy-1+ T cells in mice likely reflect developmental regulation of posttranslational modification of the Thy-1 glycoprotein. The ability of naive CD4+ T cells to respond to anti-Thy-1 stimulation increases from neonate to adult, and 6C10- naive cells from adult mice respond poorly compared with 6C10+ cells, similar to the cells in neonatal mice. These results suggest that there is functional maturation by peripheral CD4+ T cells that coincides with 6C10 glycosylated Thy-1 up-regulation, and natural autoantibody recognizes this 6C10 carbohydrate epitope.
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| pubmed:grant | |
| pubmed:language |
eng
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| pubmed:journal | |
| pubmed:citationSubset |
AIM
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| pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/Antigens, Thy-1,
http://linkedlifedata.com/resource/pubmed/chemical/Autoantibodies,
http://linkedlifedata.com/resource/pubmed/chemical/Carbohydrates,
http://linkedlifedata.com/resource/pubmed/chemical/Epitopes, T-Lymphocyte,
http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins,
http://linkedlifedata.com/resource/pubmed/chemical/Protein Isoforms
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| pubmed:status |
MEDLINE
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| pubmed:month |
Nov
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| pubmed:issn |
0022-1767
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
163
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4796-804
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| pubmed:dateRevised |
2011-11-17
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| pubmed:meshHeading |
pubmed-meshheading:10528179-Animals,
pubmed-meshheading:10528179-Antigens, Thy-1,
pubmed-meshheading:10528179-Autoantibodies,
pubmed-meshheading:10528179-Binding Sites, Antibody,
pubmed-meshheading:10528179-CD4-Positive T-Lymphocytes,
pubmed-meshheading:10528179-Carbohydrates,
pubmed-meshheading:10528179-Cell Differentiation,
pubmed-meshheading:10528179-Cell Line,
pubmed-meshheading:10528179-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10528179-Epitopes, T-Lymphocyte,
pubmed-meshheading:10528179-Glycoproteins,
pubmed-meshheading:10528179-Immunophenotyping,
pubmed-meshheading:10528179-Mice,
pubmed-meshheading:10528179-Mice, Inbred AKR,
pubmed-meshheading:10528179-Mice, Inbred BALB C,
pubmed-meshheading:10528179-Mice, Inbred C57BL,
pubmed-meshheading:10528179-Mice, Transgenic,
pubmed-meshheading:10528179-Molecular Weight,
pubmed-meshheading:10528179-Precipitin Tests,
pubmed-meshheading:10528179-Protein Isoforms,
pubmed-meshheading:10528179-Transfection,
pubmed-meshheading:10528179-Up-Regulation
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| pubmed:year |
1999
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| pubmed:articleTitle |
Peripheral CD4+ T cell maturation recognized by increased expression of Thy-1/CD90 bearing the 6C10 carbohydrate epitope.
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| pubmed:affiliation |
Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, PA 19111, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|