Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
9
pubmed:dateCreated
1999-11-19
pubmed:abstractText
DNA vaccines can stimulate both humoral and cytolytic immune responses. Although bone marrow-derived elements present the expressed Ag, the mechanisms for acquiring immunogenic peptides have yet to be fully elucidated. APCs may become directly transfected by plasmid DNA or process extracellular proteins produced by other transfected cells. Using a transactivating plasmid system and bone marrow chimeras, we show that both mechanisms appear to be involved; however, the bulk of the immune response is dependent on expression of Ag by nonlymphoid tissues and transfer to APCs. These in vivo studies are the first to define the role of transfected nonlymphoid cells in generating Ag for presentation by bone marrow-derived APCs after needle injection with plasmid DNA.
pubmed:grant
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
AIM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0022-1767
pubmed:author
pubmed:issnType
Print
pubmed:day
1
pubmed:volume
163
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
4721-7
pubmed:dateRevised
2008-11-21
pubmed:meshHeading
pubmed-meshheading:10528170-Animals, pubmed-meshheading:10528170-Antibodies, Antinuclear, pubmed-meshheading:10528170-Antigens, Surface, pubmed-meshheading:10528170-Bone Marrow, pubmed-meshheading:10528170-Cell Membrane, pubmed-meshheading:10528170-Cytotoxicity, Immunologic, pubmed-meshheading:10528170-Injections, Intradermal, pubmed-meshheading:10528170-Macrophages, pubmed-meshheading:10528170-Mice, pubmed-meshheading:10528170-Mice, Inbred BALB C, pubmed-meshheading:10528170-Mice, Inbred C57BL, pubmed-meshheading:10528170-Mice, Transgenic, pubmed-meshheading:10528170-Ovalbumin, pubmed-meshheading:10528170-Plasmids, pubmed-meshheading:10528170-Promoter Regions, Genetic, pubmed-meshheading:10528170-Skin, pubmed-meshheading:10528170-Spleen, pubmed-meshheading:10528170-T-Lymphocytes, Cytotoxic, pubmed-meshheading:10528170-Tetracycline, pubmed-meshheading:10528170-Trans-Activators, pubmed-meshheading:10528170-Transfection, pubmed-meshheading:10528170-Vaccines, DNA
pubmed:year
1999
pubmed:articleTitle
In vivo priming by DNA injection occurs predominantly by antigen transfer.
pubmed:affiliation
Department of Medicine, The Sam and Rose Stein Institute for Research on Aging, University of California at San Diego, La Jolla 92093, USA. mcorr@ucsd.edu
pubmed:publicationType
Journal Article, Research Support, U.S. Gov't, P.H.S., Research Support, Non-U.S. Gov't