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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
1
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pubmed:dateCreated |
1999-12-9
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pubmed:abstractText |
Insofar as Ca(2+) plays a major role in T cell activation, we investigated the effect of the immunosuppressants cyclosporin A and rapamycin on T cell proliferation and on the activation-induced increase in [Ca(2+)](i). Both cyclosporin A and rapamycin inhibited mitogen (concanavalin A and phytohemagglutinin) and ionomycin+phorbol myristate acetate (PMA)-driven T cell proliferation (Ca(2+)-dependent). However, only rapamycin suppressed T cell proliferation stimulated by anti-CD28 antibody (Ab)+PMA, and recombinant interleukin-6-stimulated proliferation of the interleukin-6 dependent B9 cells (Ca(2+)-independent). These differences were associated with a different effect of both drugs on Ca(2+) release, as cyclosporin A attenuated while rapamycin augmented the mitogen-induced elevation in [Ca(2+)](i). Collectively, this supports the notion that Ca(2+) is required in early stages of T cell activation, and that cyclosporin A blocked only Ca(2+)-dependent while rapamycin blocked both Ca(2+)-dependent and -independent events of T cell activation.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0014-2999
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
17
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pubmed:volume |
381
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
51-6
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10528133-Animals,
pubmed-meshheading:10528133-Calcium,
pubmed-meshheading:10528133-Cell Division,
pubmed-meshheading:10528133-Cell Line,
pubmed-meshheading:10528133-Chelating Agents,
pubmed-meshheading:10528133-Concanavalin A,
pubmed-meshheading:10528133-Cyclosporine,
pubmed-meshheading:10528133-Dose-Response Relationship, Drug,
pubmed-meshheading:10528133-Egtazic Acid,
pubmed-meshheading:10528133-Humans,
pubmed-meshheading:10528133-Immunosuppressive Agents,
pubmed-meshheading:10528133-Ionomycin,
pubmed-meshheading:10528133-Kinetics,
pubmed-meshheading:10528133-Leukocytes, Mononuclear,
pubmed-meshheading:10528133-Lymphocyte Activation,
pubmed-meshheading:10528133-Lymphocytes,
pubmed-meshheading:10528133-Sirolimus,
pubmed-meshheading:10528133-Tetradecanoylphorbol Acetate,
pubmed-meshheading:10528133-Time Factors
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pubmed:year |
1999
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pubmed:articleTitle |
Opposing effects of rapamycin and cyclosporin A on activation-induced Ca(2+) release.
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pubmed:affiliation |
Molecular Biology Section, Department of Laboratory Medicine, St. Georges Hospital, Beirut, Lebanon. wyalmawi@aub.edu.lb
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pubmed:publicationType |
Journal Article,
Research Support, Non-U.S. Gov't
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