10525536

pubmed:Citation

2

We have used microinjection and time-lapse video microscopy to study the role of cyclin A in mitosis. We have injected purified, active cyclin A/cyclin-dependent kinase 2 (CDK2) into synchronized cells at specific points in the cell cycle and assayed its effect on cell division. We find that cyclin A/CDK2 will drive G2 phase cells into mitosis within 30 min of microinjection, up to 4 h before control cells enter mitosis. Often this premature mitosis is abnormal; the chromosomes do not completely condense and daughter cells fuse. Remarkably, microinjecting cyclin A/CDK2 into S phase cells has no effect on progress through the following G2 phase or mitosis. In complementary experiments we have microinjected the amino terminus of p21(Cip1/Waf1/Sdi1) (p21N) into cells to inhibit cyclin A/CDK2 activity. We find that p21N will prevent S phase or G2 phase cells from entering mitosis, and will cause early prophase cells to return to interphase. These results suggest that cyclin A/CDK2 is a rate-limiting component required for entry into mitosis, and for progress through mitosis until late prophase. They also suggest that cyclin A/CDK2 may be the target of the recently described prophase checkpoint.

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http://linkedlifedata.com/resource/pubmed/journal/0375356

http://linkedlifedata.com/resource/pubmed/chemical/CDC2-CDC28 Kinases, http://linkedlifedata.com/resource/pubmed/chemical/CDK2 protein, human, http://linkedlifedata.com/resource/pubmed/chemical/Cyclin A, http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinase 2, http://linkedlifedata.com/resource/pubmed/chemical/Cyclin-Dependent Kinases, http://linkedlifedata.com/resource/pubmed/chemical/Protein-Serine-Threonine Kinases

Oct

pubmed-author:FurunoNN, pubmed-author:PinesJJ, pubmed-author:den ElzenNN

18

NLM

295-306

pubmed-meshheading:10525536-CDC2-CDC28 Kinases, pubmed-meshheading:10525536-Cyclin A, pubmed-meshheading:10525536-Cyclin-Dependent Kinase 2, pubmed-meshheading:10525536-Cyclin-Dependent Kinases, pubmed-meshheading:10525536-HeLa Cells, pubmed-meshheading:10525536-Humans, pubmed-meshheading:10525536-Microinjections, pubmed-meshheading:10525536-Microscopy, Video, pubmed-meshheading:10525536-Mitosis, pubmed-meshheading:10525536-Prophase, pubmed-meshheading:10525536-Protein-Serine-Threonine Kinases

Human cyclin A is required for mitosis until mid prophase.

Journal Article, Research Support, Non-U.S. Gov't