Linked Life Data

10525364

Source:http://linkedlifedata.com/resource/pubmed/id/10525364

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
1999-11-5
pubmed:abstractText
It is well documented that the pituitary in teleosts produces two gonadotropins, namely gonadotropin-I (GTH-I) and gonadotropin-II (GTH-II), which may regulate different phases of the reproductive cycle. However, unlike in mammals, very little is known about the differential regulation of the two GTHs in fish. Using goldfish as a model, the present study demonstrates, for the first time, that activin, a protein factor that plays a critical role in the differential regulation of mammalian FSH and LH, has opposite effects on GTH-Ibeta and GTH-IIbeta mRNA expression. Recombinant goldfish activin B stimulates GTH-Ibeta but significantly suppresses GTH-IIbeta mRNA levels in a dose-dependent manner in cultured goldfish pituitary cells. Administration of recombinant human follistatin completely abolished the effects of activin, thus demonstrating the specificity of the activin activities. The novel opposite effects of activin on the two goldfish GTHs make goldfish a very unique vertebrate model for activin studies. The present study not only contributes to our understanding of the mechanisms that control the temporal expression patterns of the two GTHs during the fish reproductive cycle, but also provides important information on the evolution of gonadotropin regulation in vertebrates.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Activins, http://linkedlifedata.com/resource/pubmed/chemical/DNA Probes, http://linkedlifedata.com/resource/pubmed/chemical/Follistatin, http://linkedlifedata.com/resource/pubmed/chemical/Glycoproteins, http://linkedlifedata.com/resource/pubmed/chemical/Gonadotropins, Pituitary, http://linkedlifedata.com/resource/pubmed/chemical/Oligopeptides, http://linkedlifedata.com/resource/pubmed/chemical/Peptides, http://linkedlifedata.com/resource/pubmed/chemical/RNA, Messenger, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Proteins, http://linkedlifedata.com/resource/pubmed/chemical/activin B, http://linkedlifedata.com/resource/pubmed/chemical/gonadotropin- pituitary..., http://linkedlifedata.com/resource/pubmed/chemical/myelopeptides
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0016-6480
pubmed:author
pubmed:copyrightInfo
Copyright 1999 Academic Press.
pubmed:issnType
Print
pubmed:volume
116
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
81-9
pubmed:dateRevised
2008-9-4
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Recombinant goldfish activin B stimulates gonadotropin-Ibeta but inhibits gonadotropin-IIbeta expression in the goldfish, Carassius auratus.
pubmed:affiliation
Department of Biology, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't