Linked Life Data

10525193

Source:http://linkedlifedata.com/resource/pubmed/id/10525193

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-8-16
pubmed:databankReference
pubmed:abstractText
We have analyzed the expression and intracellular distribution, during oogenesis and embryogenesis, of Vg1 RBP, a protein implicated in the intracellular localization of Vg1 mRNA to the vegetal cortex of Xenopus oocytes. Vg1 RBP (protein) colocalizes with Vg1 RNA at all stages of oogenesis. Vg1 RBP RNA, however, localizes to the animal pole during late oogenesis, and remains in the animal blastomeres and ectodermal precursors until its zygotic transcription is activated, around stage 12. Vg1 RBP mRNA then becomes expressed throughout the neural epithelium. Vg1 RBP mRNA expression is also detected in what appears to be neural crest cells undergoing delamination and lateral migration. By tailbud stages, Vg1 RBP expression is present in the branchial arches, otic vesicle, pronephros, and along the neural tube. To examine the expression pattern in different species, we cloned the zebrafish homolog of Vg1 RBP by using a highly homologous EST clone to screen an embryonic cDNA library. In situ hybridization reveals that Vg1 RBP RNA localizes early in oogenesis to the animal pole. Although Vg1 RBP RNA is detected in all blastomeres of the early embryo, the expression pattern in the one day old zebrafish embryo is almost identical to that of the equivalent stage Xenopus embryo. These results indicate that the zygotic expression pattern is similar in frogs and fish, and that there is a conserved zygotic expression of Vg1 RBP distinct from its expression in the oocyte.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0925-4773
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
101-6
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10525193-Amino Acid Sequence, pubmed-meshheading:10525193-Animals, pubmed-meshheading:10525193-Cloning, Molecular, pubmed-meshheading:10525193-Conserved Sequence, pubmed-meshheading:10525193-Embryo, Nonmammalian, pubmed-meshheading:10525193-Evolution, Molecular, pubmed-meshheading:10525193-Female, pubmed-meshheading:10525193-Gene Expression Regulation, Developmental, pubmed-meshheading:10525193-Glycoproteins, pubmed-meshheading:10525193-Molecular Sequence Data, pubmed-meshheading:10525193-Oocytes, pubmed-meshheading:10525193-RNA-Binding Proteins, pubmed-meshheading:10525193-Transforming Growth Factor beta, pubmed-meshheading:10525193-Xenopus, pubmed-meshheading:10525193-Xenopus Proteins, pubmed-meshheading:10525193-Zebrafish, pubmed-meshheading:10525193-Zebrafish Proteins
pubmed:year
1999
pubmed:articleTitle
Vg1 RBP intracellular distribution and evolutionarily conserved expression at multiple stages during development.
pubmed:affiliation
Department of Anatomy and Cell Biology, Hebrew University, Hadassah Medical School, Jerusalem, Israel.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't