Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
1
pubmed:dateCreated
2000-8-16
pubmed:abstractText
The leucine zipper transcription factors cAMP response element binding protein (CREB), cAMP response element modulatory protein (CREM) and activating transcription factor 1 (ATF1) bind to the cAMP response element (CRE) with the palindromic consensus sequence TGACGTCA. Their transcriptional activities are dependent on serine phosphorylation induced by various extracellular signals such as hormones, growth factors and neurotransmitters. Here we show that CREB is the predominant CRE-binding protein in Xenopus embryos and that it plays an essential role during early development. The importance of CREB for morphogenetic processes was assessed by injection of RNA encoding a dominant-negative form of CREB that is fused to a truncated progesterone receptor ligand binding domain. In this fusion protein, a dominant-negative function can be induced by application of the synthetic steroid RU486 at given developmental stages. The inhibition of CREB at blastula and early gastrula stages leads to severe posterior defects of the embryos reflected by strong spina bifida, whereas the inhibition of CREB at the beginning of neurulation resulted in stunted embryos with microcephaly. In these embryos, initial induction of neural and mesodermal tissues is not dependent on CREB function, as genes such as Otx2, Krox20, Shh and MyoD are still expressed in injected embryos. But the expression domains of Otx2 and MyoD were found to be distorted reflecting the abnormal development in both neural and somitic derivatives. In summary, our data show that CREB is essential during several developmental stages of Xenopus embryogenesis.
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
http://linkedlifedata.com/resource/pubmed/chemical/Acetyltransferases, http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response Element..., http://linkedlifedata.com/resource/pubmed/chemical/Cyclic AMP Response..., http://linkedlifedata.com/resource/pubmed/chemical/DNA-Binding Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Early Growth Response Protein 2, http://linkedlifedata.com/resource/pubmed/chemical/Egr2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Genetic Markers, http://linkedlifedata.com/resource/pubmed/chemical/Hedgehog Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Homeodomain Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Hormone Antagonists, http://linkedlifedata.com/resource/pubmed/chemical/Krox-20 protein, Xenopus, http://linkedlifedata.com/resource/pubmed/chemical/Mifepristone, http://linkedlifedata.com/resource/pubmed/chemical/MyoD Protein, http://linkedlifedata.com/resource/pubmed/chemical/Nerve Tissue Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Otx Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Otx2 protein, Xenopus, http://linkedlifedata.com/resource/pubmed/chemical/Otx2 protein, mouse, http://linkedlifedata.com/resource/pubmed/chemical/Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Receptors, Progesterone, http://linkedlifedata.com/resource/pubmed/chemical/Recombinant Fusion Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Repressor Proteins, http://linkedlifedata.com/resource/pubmed/chemical/Trans-Activators, http://linkedlifedata.com/resource/pubmed/chemical/Transcription Factors, http://linkedlifedata.com/resource/pubmed/chemical/Xenopus Proteins, http://linkedlifedata.com/resource/pubmed/chemical/alcohol O-acetyltransferase
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0925-4773
pubmed:author
pubmed:issnType
Print
pubmed:volume
88
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
55-66
pubmed:dateRevised
2006-11-15
pubmed:meshHeading
pubmed-meshheading:10525188-Acetyltransferases, pubmed-meshheading:10525188-Amino Acid Sequence, pubmed-meshheading:10525188-Animals, pubmed-meshheading:10525188-Blastocyst, pubmed-meshheading:10525188-Cyclic AMP Response Element Modulator, pubmed-meshheading:10525188-Cyclic AMP Response Element-Binding Protein, pubmed-meshheading:10525188-DNA-Binding Proteins, pubmed-meshheading:10525188-Early Growth Response Protein 2, pubmed-meshheading:10525188-Embryo, Nonmammalian, pubmed-meshheading:10525188-Gastrula, pubmed-meshheading:10525188-Gene Expression Regulation, Developmental, pubmed-meshheading:10525188-Genes, Dominant, pubmed-meshheading:10525188-Genetic Markers, pubmed-meshheading:10525188-Hedgehog Proteins, pubmed-meshheading:10525188-Homeodomain Proteins, pubmed-meshheading:10525188-Hormone Antagonists, pubmed-meshheading:10525188-Mice, pubmed-meshheading:10525188-Microinjections, pubmed-meshheading:10525188-Mifepristone, pubmed-meshheading:10525188-Molecular Sequence Data, pubmed-meshheading:10525188-MyoD Protein, pubmed-meshheading:10525188-Nerve Tissue Proteins, pubmed-meshheading:10525188-Otx Transcription Factors, pubmed-meshheading:10525188-Phenotype, pubmed-meshheading:10525188-Proteins, pubmed-meshheading:10525188-Receptors, Progesterone, pubmed-meshheading:10525188-Recombinant Fusion Proteins, pubmed-meshheading:10525188-Repressor Proteins, pubmed-meshheading:10525188-Trans-Activators, pubmed-meshheading:10525188-Transcription Factors, pubmed-meshheading:10525188-Xenopus, pubmed-meshheading:10525188-Xenopus Proteins
pubmed:year
1999
pubmed:articleTitle
Essential role of CREB family proteins during Xenopus embryogenesis.
pubmed:affiliation
Max-Planck-Institute of Psychiatry, Munich, Germany. lutz@mpipsykl.mpg.de
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't