Linked Life Data

10523574

Source:http://linkedlifedata.com/resource/pubmed/id/10523574

Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:issue
11
pubmed:dateCreated
1999-11-30
pubmed:databankReference
pubmed:abstractText
Because identification of the species within the "Streptococcus milleri" group is difficult for the clinical laboratory as the species share overlapping phenotypic characteristics, we wished to confirm biochemical identification with identification by 16S rRNA gene sequence analysis. Ninety-four clinical isolates previously identified as the "Streptococcus milleri" group were reclassified as S. anginosus, S. constellatus, or S. intermedius with the API 20 Strep system (bioMerieux Vikek, Hazelton, Mo.) and the Fluo-card (Key Scientific, Round Rock, Tex.). In addition, we determined the Lancefield group, hemolysis, colony size, colony texture, repetitive extragenic palindromic PCR (rep-PCR) pattern, and cellular fatty acid (CFA) profile (MIDI, Newark, Del.). 16S rRNA gene sequence analysis with 40 selected representative strains showed three distinct groups, with S. constellatus and S. intermedius found to be more closely related to each other than to S. anginosus, and further distinguished a biochemically distinct group of urogenital isolates within the S. anginosus group of isolates. Except for strains unreactive with the Fluo-card (8%), all S. anginosus and S. intermedius strains identified by sequencing were similarly identified by biochemical testing. However, 23% of the selected S. constellatus isolates identified by sequencing (9% of all S. constellatus isolates) would have been identified as S. anginosus or S. intermedius by biochemical tests. Although most S. anginosus strains formed one unique cluster by CFA analysis and most S. constellatus strains showed similar rep-PCR patterns, neither method was sufficiently dependable for identification. Whereas Lancefield group or lactose fermentation did not correspond to sequence or biochemical type, S. constellatus was most likely to be beta-hemolytic and S. intermedius was most likely to have a dry colony type. The most frequent isolate in our population was S. constellatus, followed by S. anginosus. There was an association of S. anginosus with a gastrointestinal or urogenital source, and there was an association of S. constellatus and S. intermedius with both the respiratory tract and upper-body abscesses.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-1734062, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-1995029, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-2380375, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-3060239, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-7537057, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-7545512, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-7559957, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-7572815, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-7650193, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-7803636, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-7805358, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-8567909, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-8784576, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-8818904, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523574-9276413
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Nov
pubmed:issn
0095-1137
pubmed:author
pubmed:issnType
Print
pubmed:volume
37
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
3681-7
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Genotypic and phenotypic characterization of "Streptococcus milleri" group isolates from a Veterans Administration hospital population.
pubmed:affiliation
Department of Pathology, Baylor College of Medicine, Veterans Affairs Medical Center, Houston, Texas 77030, USA. jillc@bcm.tmc.edu
pubmed:publicationType
Journal Article, Comparative Study