Statements in which the resource exists as a subject.
PredicateObject
rdf:type
lifeskim:mentions
pubmed:dateCreated
1999-12-15
pubmed:abstractText
1. Intracellular free calcium concentration ([Ca2+]i) was measured with the fluorescent indicator indo-1 in single skeletal fibres enzymatically isolated from the flexor digitorum brevis and interosseus muscles of control and dystrophic mdx C57BL/10 mice. Measurements were taken from a portion of fibre that was voltage clamped to allow detection of depolarization-induced changes in [Ca2+]i. 2. The mean (+/- s.e.m.) initial resting [Ca2+]i from all control and mdx fibres tested was 56 +/- 5 nM (n = 72) and 48 +/- 7 nM (n = 57), respectively, indicating no significant overall difference between the two groups. However, when comparing a batch of control and mdx fibres obtained from mice older than approximately 35 weeks, resting [Ca2+]i was significantly lower in mdx (16 +/- 4 nM, n = 11) than in control fibres (71 +/- 10 nM, n = 14). 3. Changes in [Ca2+]i elicited by short (5-35 ms) depolarizing pulses from -80 to 0 mV showed similar properties in control and mdx fibres. After a 5 ms duration pulse the mean time constant of [Ca2+]i decay was, however, significantly elevated in mdx as compared to control fibres, by a factor of 1.5-2. For longer pulses, no significant difference could be detected. 4. In response to 50 ms duration depolarizing pulses of various amplitudes the threshold for detection of an [Ca2+]i change and the peak [Ca2+]i reached for a given potential were similar in control and mdx fibres. 5. Overall results show that mdx skeletal muscle fibres are quite capable of handling [Ca2+]i at rest and in response to membrane depolarizations.
pubmed:commentsCorrections
http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-10066910, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-1512554, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-1661733, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-1936568, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-2173137, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-2447503, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-3173492, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-3209690, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-3290684, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-3395664, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-3612180, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-3838314, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-6583703, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-7742348, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-8271194, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-8358771, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-8487190, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-8567942, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-8645705, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-8729700, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-8762085, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-8897840, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-9251808, http://linkedlifedata.com/resource/pubmed/commentcorrection/10523411-9675196
pubmed:language
eng
pubmed:journal
pubmed:citationSubset
IM
pubmed:chemical
pubmed:status
MEDLINE
pubmed:month
Oct
pubmed:issn
0022-3751
pubmed:author
pubmed:issnType
Print
pubmed:day
15
pubmed:volume
520 Pt 2
pubmed:owner
NLM
pubmed:authorsComplete
Y
pubmed:pagination
417-29
pubmed:dateRevised
2009-11-18
pubmed:meshHeading
pubmed:year
1999
pubmed:articleTitle
Intracellular calcium signals measured with indo-1 in isolated skeletal muscle fibres from control and mdx mice.
pubmed:affiliation
Laboratoire de Physiologie des Elements Excitables, CNRS UMR 5578, Universite Claude Bernard Lyon 1, 43 boulevard du 11 Novembre 1918, 69622 Villeurbanne Cedex, France.
pubmed:publicationType
Journal Article, Research Support, Non-U.S. Gov't