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rdf:type |
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lifeskim:mentions |
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pubmed:issue |
6752
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pubmed:dateCreated |
1999-10-22
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pubmed:databankReference |
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pubmed:abstractText |
In most arterial beds a significant endothelium-dependent dilation to various stimuli persists even after inhibition of nitric oxide synthase and cyclo-oxygenase. This dilator response is preceded by an endothelium-dependent hyperpolarization of vascular smooth muscle cells, which is sensitive to a combination of the calcium-dependent potassium-channel inhibitors charybdotoxin and apamin, and is assumed to be mediated by an unidentified endothelium-derived hyperpolarizing factor (EDHF). Here we show that the induction of cytochrome P450 (CYP) 2C8/34 in native porcine coronary artery endothelial cells by beta-naphthoflavone enhances the formation of 11,12-epoxyeicosatrienoic acid, as well as EDHF-mediated hyperpolarization and relaxation. Transfection of coronary arteries with CYP 2C8/34 antisense oligonucleotides results in decreased levels of CYP 2C and attenuates EDHF-mediated vascular responses. Thus, a CYP-epoxygenase product is an essential component of EDHF-mediated relaxation in the porcine coronary artery, and CYP 2C8/34 fulfils the criteria for the coronary EDHF synthase.
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pubmed:language |
eng
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pubmed:journal |
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pubmed:citationSubset |
IM
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pubmed:chemical |
http://linkedlifedata.com/resource/pubmed/chemical/11,12-epoxy-5,8,14-eicosatrienoic...,
http://linkedlifedata.com/resource/pubmed/chemical/8,11,14-Eicosatrienoic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Arachidonic Acid,
http://linkedlifedata.com/resource/pubmed/chemical/Biological Factors,
http://linkedlifedata.com/resource/pubmed/chemical/Bradykinin,
http://linkedlifedata.com/resource/pubmed/chemical/Cytochrome P-450 Enzyme System,
http://linkedlifedata.com/resource/pubmed/chemical/Oligonucleotides, Antisense,
http://linkedlifedata.com/resource/pubmed/chemical/Oxygenases,
http://linkedlifedata.com/resource/pubmed/chemical/endothelium-dependent...
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pubmed:status |
MEDLINE
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pubmed:month |
Sep
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pubmed:issn |
0028-0836
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pubmed:author |
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pubmed:issnType |
Print
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pubmed:day |
30
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pubmed:volume |
401
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pubmed:owner |
NLM
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pubmed:authorsComplete |
Y
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pubmed:pagination |
493-7
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pubmed:dateRevised |
2006-11-15
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pubmed:meshHeading |
pubmed-meshheading:10519554-8,11,14-Eicosatrienoic Acid,
pubmed-meshheading:10519554-Animals,
pubmed-meshheading:10519554-Arachidonic Acid,
pubmed-meshheading:10519554-Biological Factors,
pubmed-meshheading:10519554-Bradykinin,
pubmed-meshheading:10519554-Cells, Cultured,
pubmed-meshheading:10519554-Coronary Vessels,
pubmed-meshheading:10519554-Cytochrome P-450 Enzyme System,
pubmed-meshheading:10519554-Endothelium, Vascular,
pubmed-meshheading:10519554-Enzyme Induction,
pubmed-meshheading:10519554-Humans,
pubmed-meshheading:10519554-Molecular Sequence Data,
pubmed-meshheading:10519554-Oligonucleotides, Antisense,
pubmed-meshheading:10519554-Oxygenases,
pubmed-meshheading:10519554-Reverse Transcriptase Polymerase Chain Reaction,
pubmed-meshheading:10519554-Swine,
pubmed-meshheading:10519554-Vasodilation
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pubmed:year |
1999
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pubmed:articleTitle |
Cytochrome P450 2C is an EDHF synthase in coronary arteries.
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pubmed:affiliation |
Institut für Kardiovaskuläre Physiologie, Klinikum der J.W. Goethe-Universität, Frankfurt am Main, Germany.
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pubmed:publicationType |
Journal Article,
In Vitro,
Research Support, Non-U.S. Gov't
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