Source:http://linkedlifedata.com/resource/pubmed/id/10519414
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| Predicate | Object |
|---|---|
| rdf:type | |
| lifeskim:mentions | |
| pubmed:issue |
19
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| pubmed:dateCreated |
1999-11-4
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| pubmed:abstractText |
Uridine phosphorylase (UPase) plays an important role in the activation of 5-fluorouracil and in the regulation of tissue and plasma concentration of uridine, a potential biochemical modulator of 5-fluorouracil therapy. UPase expression is affected by the c-H-ras oncogene and various cytokines through unknown mechanisms. To understand its expression and regulation, we cloned the murine UPase gene, defined its genomic organization, determined its 5'- and 3'-end flanking sequences, and evaluated the promoter activity. The UPase gene contains nine exons and eight introns, spanning a total of approximately 18.0 kb. Its promoter lacks canonical TATA and CCAAT boxes, although a CAATAAAAA TATA-like box is seen from -41 to -49. Furthermore, IFN regulatory factor 1, c/v-Myb, and p53 binding sites are present in the promoter region, indicating that UPase expression may be directly regulated by cytokines and oncogene products. The 1.2-kb flanking fragment showed promoter activity driving the expression of the luciferase gene in various mammalian cells. A TGGGG repeat sequence is seen in the 3'-end flanking region. This element is considered to be a potential recombination consensus hot spot that may contribute to the encoding of different UPase isoforms present in different tissues, both normal and neoplastic.
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| pubmed:grant | |
| pubmed:language |
eng
|
| pubmed:journal | |
| pubmed:citationSubset |
IM
|
| pubmed:chemical | |
| pubmed:status |
MEDLINE
|
| pubmed:month |
Oct
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| pubmed:issn |
0008-5472
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| pubmed:author | |
| pubmed:issnType |
Print
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| pubmed:day |
1
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| pubmed:volume |
59
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| pubmed:owner |
NLM
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| pubmed:authorsComplete |
Y
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| pubmed:pagination |
4997-5001
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| pubmed:dateRevised |
2008-11-21
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| pubmed:meshHeading |
pubmed-meshheading:10519414-3' Untranslated Regions,
pubmed-meshheading:10519414-5' Untranslated Regions,
pubmed-meshheading:10519414-Animals,
pubmed-meshheading:10519414-Base Sequence,
pubmed-meshheading:10519414-Binding Sites,
pubmed-meshheading:10519414-Chromosome Mapping,
pubmed-meshheading:10519414-Cloning, Molecular,
pubmed-meshheading:10519414-DNA-Binding Proteins,
pubmed-meshheading:10519414-Exons,
pubmed-meshheading:10519414-Gene Expression Regulation, Enzymologic,
pubmed-meshheading:10519414-Introns,
pubmed-meshheading:10519414-Karyotyping,
pubmed-meshheading:10519414-Lymphocytes,
pubmed-meshheading:10519414-Mice,
pubmed-meshheading:10519414-Molecular Sequence Data,
pubmed-meshheading:10519414-Polymerase Chain Reaction,
pubmed-meshheading:10519414-Promoter Regions, Genetic,
pubmed-meshheading:10519414-Restriction Mapping,
pubmed-meshheading:10519414-Spleen,
pubmed-meshheading:10519414-Uridine Phosphorylase
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| pubmed:year |
1999
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| pubmed:articleTitle |
Genomic structure, chromosomal mapping, and promoter region analysis of murine uridine phosphorylase gene.
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| pubmed:affiliation |
Department of Internal Medicine (Oncology), Yale University School of Medicine, New Haven, Connecticut 06520, USA.
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| pubmed:publicationType |
Journal Article,
Research Support, U.S. Gov't, P.H.S.
|