rdf:type |
|
lifeskim:mentions |
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pubmed:issue |
2
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pubmed:dateCreated |
1999-11-17
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pubmed:abstractText |
HPLC analysis of the amino acid contents of the second- and third-order giant fibres at the giant synapse in the stellate ganglion of the squid Loligo vulgaris shows that there are significantly higher amounts of L-glutamate and L-aspartate in the second-order (presynaptic) fibre than in the third-order (postsynaptic) fibre. Immunocytochemical staining of sections of the ganglion with an antibody raised against L-glutamate produces specific positive staining in the synaptic region of the second-order fibre. In contrast, staining with antibodies raised against glutamate-receptors (mammalian GluR1 with GluR2/3) produces positive staining in the third-order fibre at the postsynaptic region. These data provide further evidence for the hypothesis that L-glutamate is an excitatory transmitter at the giant synapse.
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pubmed:language |
eng
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pubmed:journal |
|
pubmed:citationSubset |
IM
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pubmed:chemical |
|
pubmed:status |
MEDLINE
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pubmed:month |
Aug
|
pubmed:issn |
0006-8993
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pubmed:author |
|
pubmed:issnType |
Print
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pubmed:day |
28
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pubmed:volume |
839
|
pubmed:owner |
NLM
|
pubmed:authorsComplete |
Y
|
pubmed:pagination |
213-20
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pubmed:dateRevised |
2005-11-17
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pubmed:meshHeading |
pubmed-meshheading:10519043-Alanine,
pubmed-meshheading:10519043-Animals,
pubmed-meshheading:10519043-Antibody Specificity,
pubmed-meshheading:10519043-Aspartic Acid,
pubmed-meshheading:10519043-Blotting, Western,
pubmed-meshheading:10519043-Chromatography, High Pressure Liquid,
pubmed-meshheading:10519043-Decapodiformes,
pubmed-meshheading:10519043-Electrophoresis, Polyacrylamide Gel,
pubmed-meshheading:10519043-Fluorescent Antibody Technique,
pubmed-meshheading:10519043-Ganglia, Invertebrate,
pubmed-meshheading:10519043-Glutamic Acid,
pubmed-meshheading:10519043-Glycine,
pubmed-meshheading:10519043-Receptors, Glutamate,
pubmed-meshheading:10519043-Serine,
pubmed-meshheading:10519043-Synapses,
pubmed-meshheading:10519043-Taurine,
pubmed-meshheading:10519043-gamma-Aminobutyric Acid
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pubmed:year |
1999
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pubmed:articleTitle |
Localization of L-glutamate and glutamate-like receptors at the squid giant synapse.
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pubmed:affiliation |
Dipartimento di Zoologia, Università degli Studi Federico II, Naples, Italy.
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pubmed:publicationType |
Journal Article
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